Synaptotagmin 1 (pSer309) Antibody

Synaptotagmin 1 (pSer309) Antibody gallery image 1

Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~60 kDa and ~62 kDa synaptotagmin phosphorylated at Se369

  • Synaptotagmin 1 (pSer309) Antibody thumbnail image 1
  • Rabbit anti Synaptotagmin 1 (pSer309)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1239WB*datasheet pdfdatasheet pdf0.1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Rat synaptotagmin 1 (pSer309) antibody recognizes synaptotagmin 1, also known as p65, when phosphorylated at serine 309. Synaptotagmin 1 is a ~60 kDa member of the synaptotagmin family, expressed in membranes of synaptic vesicles in rostral, phylogenetically younger brain regions and in some endocrine tissues. Ca2+ is bound by synaptogamin whose C2 domains then bind phospholipids, trafficking vesicles to the synaptic membrane and exocytosis. This results in fast synchronous neurotransmitter release. Synaptotagmin also binds neurexins, syntaxin and AP2 independently of Ca2+ binding.

      Phosphorylation of synaptotagmin by protein kinases is thought to modulate its activity.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      BovineExpected from Sequence
      ChickenExpected from Sequence
      HumanExpected from Sequence
      ZebrafishExpected from Sequence
      MouseExpected from Sequence
      DogExpected from Sequence
      PrimateExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to synaptotagmin 1 (pSer309) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.01%Bovine Serum Albumin
      50% Glycerol
    • Immunogen
      Phosphopeptide corresponding to amino acid sequence surrounding phosphorylated serine 309 of synaptotagmin 1.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM Hepes pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      regulation of calcium ion-dependent exocytosis
      synaptic vesicle exocytosis
      phospholipid binding
      transporter activity
      cell junction
      integral to membrane
      identical protein binding
      chromaffin granule membrane
      calmodulin binding
      calcium ion binding
      calcium ion-dependent exocytosis
      dense core granule
      synaptic vesicle membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1239 detects a band of approximately 60kDa in rat cortex cell lysates.
    • Instructions For Use

    Additional Synaptotagmin 1 (pSer309) Antibody Formats

    Formats Applications Sizes available
    Synaptotagmin 1 (pSer309) Antibody : Purified WB* 0.1 ml
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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