Synapsin I (pSer9) Antibody

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Synapsin I (pSer9) Antibody gallery image 1

Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~78 kDa synapsin 1 protein phosphorylated at Ser9

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  • Rabbit anti Synapsin I (pSer9)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP909WB*datasheet pdfdatasheet pdf0.1 ml
    AHP909
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti phosphorylated Synapsin I antibody recognizes rat Synapsin I, when phosphorylated at serine 9.

      Synapsin I is a neuron-specific phosphoprotein which is associated with synaptic vesicles in the central and peripheral nervous system and is involved in the regulation of synaptic vesicle availability, and thought to play a key role in brain plasticity.

      Synapsin I is phosphorylated at serine 9 by cAMP-dependent protein kinase and calcium calmodulin kinase I.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      ZebrafishExpected from Sequence
      MouseExpected from Sequence
      XenopusExpected from Sequence
      HumanExpected from Sequence
      BovineExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to phosphorylated synapsin I were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.01%Bovine Serum Albumin
      50%Glycerol
    • Immunogen
      Synthetic phosphopeptide corresponding to an amino acid sequence within Synapsin I which includes the phosphorylated residue Ser9.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM HEPES pH7.5
      150mM NaCl
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      (1)
      For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP909 detects a band of approximately 78kDa in rat cortex lysates. In some cases there may be an additional faint band at approximately 55kD, which corresponds to slight cross-reactivity with phosphorylated synapsin II.
    • Instructions For Use

    Additional Synapsin I (pSer9) Antibody Formats

    Formats Applications Sizes available
    Synapsin I (pSer9) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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