Synapsin I (pSer549) Antibody

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Synapsin I (pSer549) Antibody gallery image 1

Western blot of rat brain lysate showing phosphospecific immunolabeling of the ~78 kDa synapsin 1 protein phosphorylated at Ser549

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  • Rabbit anti Synapsin I (pSer549)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1238WB*datasheet pdfdatasheet pdf0.1 ml
    AHP1238
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Rat synapsin I (pSer549) antibody recognizes synapsin I, also known as Syn1, when phosphorylated at serine 549. Synapsin I is a ~78 kDa member of the synapsin family, expressed in the central and peripheral nervous system. Synapsins coat synaptic vesicles, binding them to the cytoskeleton and are thought to regulate their availability for release. Synapsins are important in brain plasticity, axonogenesis and synaptogenesis. Mutations in the synapsin I gene may be associated with disorders such as X-linked recessive epilepsy associated with variable learning disabilities and behavior disorders.

      Phosphorylation of serine residue 549 by MAP kinase or cyclin-dependent kinases 1 or 5 is an important part in modulating synaptic vesicle trafficking.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      HumanExpected from Sequence
      BovineExpected from Sequence
      DogExpected from Sequence
      MouseExpected from Sequence
      PrimateExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to synapsin I (pSer 549) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.01%Bovine Serum Albumin
      50% Glycerol
    • Immunogen
      Phosphopeptide corresponding to amino acid sequence surrounding the phosphorylated serine 549 of synapsin I.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM Hepes pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      (1)
      For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1238 detects a band of approximately 78kDa in rat cortex cell lysates.
    • Instructions For Use

    Additional Synapsin I (pSer549) Antibody Formats

    Formats Applications Sizes available
    Synapsin I (pSer549) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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