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RT6.2 Antibody | GY1/12

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  • Rat anti Rat RT6.2 (Peripheral T Cells)
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1157E, F, Rdatasheet pdfdatasheet pdf2 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rat anti Rat RT6.2 antibody, clone GY1/12 recognizes rat T-cell ecto-ADP-ribosyltransferase 2, also known as Alloantigen Rt6.2, RT6.2, ADP-ribosyltransferase C2 and C3 toxin-like 2, ARTC2, Mono(ADP-ribosyl) transferase 2B or T-cell surface protein Rt6.2. RT6.2 is a 275 amino acid GPI anchored cell membrane protein belonging to the Arg-specific ADP-ribosyltransferase family. RT6.2 has an N-terminal 20 amino acid signal peptide sequence and 29 amino acid C-terminal propeptide region removed in the mature form.

      Rt6 is expressed exclusively by post-thymic T cells (Koch et al. 1990) with RT6.2 alloantigen being expressed by RT6B rat strains incliuding BH.6B, BS,6B, LEW.6b and F334.6B (Koch et al. 1988). Defects in the RT6 gene expression correlate with increased susceptibility for insulin dependent diabetes and other autoimmune diseases (Haaq et al. 1996). Rat anti Rat RT6.2 antibody, clone GY1/12 does not recognize the RT6.1 alloantigen expressed by post-thymic T-cells of RT6A rat strains including BUF, LEW and PVG. The RT6.1 alloantigen is recognized by the Rat anti Rat RT6.1 antibody, clone P4/16.
    • Intended Use
    • Target Species
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
      Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Spleen and lymph node cells from F344 rats.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cell from immunised PVG-RT11 (Agus) rats were fused with cells of the Y3.Ag1.2.3 rat myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      NAD(P)+-protein-arginine ADP-ribosyltransferase activity
      anchored to membrane
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/4

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 50ul of the suggested working dilution to label 5 x 105 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional RT6.2 Antibody Formats

    Formats Clone Applications Sizes available
    RT6.2 Antibody : S/N GY1/12 E, F, R 2 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry


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