Ribosomal S6 Kinase 2 Antibody

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Ribosomal S6 Kinase 2 Antibody gallery image 1

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~90 kDa RSK2 protein

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  • Ribosomal S6 Kinase 2 Antibody thumbnail image 1
  • Rabbit anti Ribosomal S6 Kinase 2 (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1155WBdatasheet pdfdatasheet pdf0.1 ml
    AHP1155
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Rat ribosomal S6 kinase 2 antibody recognizes ribosomal S6 Kinase 2 a ~83 kDa member of the S6 kinase subfamily of Ser/Thr protein kinases. The kinase is downstream of the extracellular signal-related kinase (ERK)/MAPK cascade and mediates growth-factor and stress induced activation of the transcription factor cAMP response element-binding protein. Ribosomal S6 Kinase 2 has been shown to inhibit G protein-coupled receptors and it’s loss leads to Coffin-Lowry syndrome and nonsyndromic X-linked mental retardation. It is widely expressed, primarily in lung and skeletal muscle.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      ChickenExpected from Sequence
      HumanExpected from Sequence
      BovineExpected from Sequence
      MouseExpected from Sequence
      DogExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to rat ribosomal S6 kinase 2 (CT) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.01%Bovine Serum Albumin
      50%Glycerol
    • Immunogen
      A synthetic peptide corresponding to the C-terminal region of rat ribosomal S6 kinase 2, conjugated to keyhole limpet hemocyanin.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM Hepes pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1155 detects a band of approximately 90kDa in rat hippocampal lysates.
    • Instructions For Use

    Additional Ribosomal S6 Kinase 2 Antibody Formats

    Formats Applications Sizes available
    Ribosomal S6 Kinase 2 Antibody : Purified WB 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

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