Reticulum Cells Antibody | ED11

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  • Mouse anti Rat Reticulum Cells
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    ED11
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA733RC *, Edatasheet pdfdatasheet pdf0.25 mg
    MCA733R
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • MCA733R recognises reticular elements in the T cell areas and B cell follicles of spleen, lymph nodes, Peyers patches, bronchus and gut associated lymphoid tissues (BALT and GALT). In the thymus a reticular pattern is observed throughout the cortex capsule, the medulla is negative. In non-lymphoid organs positivity is seen only in reticular elements of the small intestine. No staining of liver, lung, skin, kidney, brain, pancreas or heart is seen.

      Biochemical analysis has revealed that ED11 recognises complement factor C3. in vivo studies have shown that ED11 blocks immune complex trapping on follicular dendritic cells.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Rat spleen cell homogenate
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with the mouse SP2/0.Ag14 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)1/1001/300
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Reticulum Cells Antibody Formats

    Formats Clone Applications Sizes available
    Reticulum Cells Antibody : Purified ED11 C *, E 0.25 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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