RANTES (CCL5) is a chemoattractant protein acting on peripheral blood monocytes and CD4/CD45RO +ve T cells.Rat RANTES demonstrates chemotactic activity on rat peritoneal macrophages in in vitro assays.
Purified recombinant protein expressed in E. coli - lyophilised
Reconstitute with 0.2 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
Protein Molecular Weight
7.9kD (69 Amino acid sequence)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.