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RAF-1 (pSer642) Antibody

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  • Rabbit anti RAF-1 (pSer642)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1237WB*datasheet pdfdatasheet pdf0.1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human phospho-Raf-1 antibody recognizes Raf-1 phosphorylated at serine 642. Raf-1is a ubiquitously expressed ~74 kDa member of the serine/threonine protein kinase family. Raf-1 is a MAP kinase kinase kinase and participates in the transduction of mitogenic signals from receptors on the cell membrane to the nucleus. Raf-1 is activated through phosphorylation by Ras and in turn phosphorylates MEK which activates ERK. ERK when activated regulates the expression of genes involved in cell cycle, differentiation, migration and apoptosis. As such Raf-1 is an important clinical target in cancer.

      Raf-1 activity can be downregulated by phosphorylation at serine residues 301 and 642.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      HumanExpected from Sequence
      BovineExpected from Sequence
      ChickenExpected from Sequence
      MouseExpected from Sequence
      DogExpected from Sequence
      PrimateExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to rat Raf-1 (pSer 642) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.01%Bovine Serum Albumin
      50% Glycerol
    • Immunogen
      Phosphopeptide corresponding to animo acid sequence surrounding phosphorylated Ser 642 of rat Raf-1.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM Hepes pH7.5
      150mM NaCl
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      protein heterodimerization activity
      mitogen-activated protein kinase kinase binding
      heart development
      ATP binding
      metal ion binding
      MAP kinase kinase kinase activity
      protein phosphorylation
      MAPKKK cascade
      response to hypoxia
      insoluble fraction
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1237 detects a band of approximately 74kDa.
    • Instructions For Use

    Additional RAF-1 (pSer642) Antibody Formats

    Formats Applications Sizes available
    RAF-1 (pSer642) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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