MHC Class II RT1Bu/L Antibody | OX-3

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MHC Class II RT1Bu/L Antibody | OX-3 gallery image 1

Published customer image:
Mouse anti Rat MHC class II antibody, clone OX-3 used to label MHC class II expressing cells in formalin fixed mouse brain cryosections by immunohistochemistry.
Image caption:
(a) Representative immunostained micrographs show Iba1+ cells in the SN of 3-, 6-, 9-, and 12-month-old female and male mice. Scale bar: 10?μm. Quantitative results of Iba1+ cell areas (b) and the number of Iba1+ cells (c) in the SN of female and male mice at different ages (n?=?5). *(p <0.05), **(p <0.01), ***(p <0.001): versus the opposite sex. (d) Representative immunostained micrographs show MHC class II+ cells in the SN of 3-, 6-, 9-, and 12-month-old female and male mice. Scale bar: 10?μm. Quantitative results of MHC class II+ cell areas (e) and the number of MHC class II+ cells (f) in the SN of female and male mice at different ages (n?=?5). ***(p <0.001): versus the opposite sex. (g) Representative immunostained micrographs show TH+ cells in the SN of 3-, 6-, 9-, and 12-month-old female and male mice. Scale bar: 200?μm. (h) Quantitative results of TH+ cells in the SN of female and male mice at different ages (n?=?5). *(p <0.05), **(p <0.01): versus the opposite sex.

From: Wu SY, Chen YW, Tsai SF, Wu SN, Shih YH, Jiang-Shieh YF, Yang TT, Kuo YM.
Estrogen ameliorates microglial activation by inhibiting the Kir2.1 inward-rectifier K(+) channel.
Sci Rep. 2016 Mar 10;6:22864.

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  • MHC Class II RT1Bu/L Antibody | OX-3 thumbnail image 1
  • Mouse anti Rat MHC Class II RT1Bu/L:RPE
  • Mouse anti Rat MHC Class II RT1Bu/L:FITC
  • Mouse anti Rat MHC Class II RT1Bu/L
  • Mouse anti Rat MHC Class II RT1Bu/L
  • Mouse anti Rat MHC Class II RT1Bu/L
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    OX-3
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA45FTFdatasheet pdfdatasheet pdf0.1 mg
    MCA45FT
    MCA45GAC, F, Pdatasheet pdfdatasheet pdf0.1 mg
    MCA45GA
    MCA45RC, F, Pdatasheet pdfdatasheet pdf0.25 mg
    MCA45R
    MCA45GC, F, Pdatasheet pdfdatasheet pdf1 mg
    MCA45G
    MCA45PEFdatasheet pdfdatasheet pdf100 Tests
    MCA45PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat MHC Class II RT1Bu/L antibody, clone OX-3 recognizes a polymorphic determinant of the rat RT1B MHC class II antigen, reacting with haplotypes u and l. The literature reports reactivity with Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. This antibody is useful for distinguishing RT1B positive cells from different rat strains, e.g. for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras.

      The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.

      Mouse anti Rat MHC Class II RT1Bu/L antibody, clone OX-3 also cross reacts with mouse strains of the H-2 haplotypes b and s. Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region.

      This product is routinely tested in flow cytometry on Lewis rat splenocytes.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by ion exchange chromatography
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography from tissue culture supernatant
      Pack Size: 1 mg, 0.1 mgPurified IgG prepared by ion exchange chromatography from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography from tissue culture supernatant
      Pack Size: 1 mg, 0.1 mgPurified IgG prepared by ion exchange chromatography from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography from tissue culture supernatant
      Pack Size: 1 mg, 0.1 mgPurified IgG prepared by ion exchange chromatography from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      Pack Size: 0.25 mg0.09% Sodium Azide (NaN3)
      Pack Size: 1 mg, 0.1 mg
      0.09%Sodium Azide
      Pack Size: 0.25 mg0.09% Sodium Azide (NaN3)
      Pack Size: 1 mg, 0.1 mg
      0.09%Sodium Azide
      Pack Size: 0.25 mg0.09% Sodium Azide (NaN3)
      Pack Size: 1 mg, 0.1 mg
      0.09%Sodium Azide
    • Immunogen
      Rat thymocyte membrane glycoproteins.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 1 mg, 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 1 mg, 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 1 mg, 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of reconstitution
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from the date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunohistology - Frozen
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunohistology - Frozen
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunohistology - Frozen
      Immunohistology - Paraffin

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin embedded sections.
      This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin embedded sections.
      This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining.

      This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections.
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional MHC Class II RT1Bu/L Antibody Formats

    Formats Clone Applications Sizes available
    MHC Class II RT1Bu/L Antibody : Purified OX-3 C, F, P 0.1 mg | 1 mg | 0.25 mg
    MHC Class II RT1Bu/L Antibody : FITC OX-3 F 0.1 mg
    MHC Class II RT1Bu/L Antibody : RPE OX-3 F 100 Tests
    • Copyright © 2016 Bio-Rad

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              References

              Further Reading

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