MHC Class I RT1Ac Antibody | OX-27

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MHC Class I RT1Ac Antibody | OX-27 gallery image 1

Staining of BN rat spleen cells with Mouse anti Rat RT1Ac (MCA156R) visualised with Goat anti Mouse IgG:FITC (STAR70)

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MHC Class I RT1Ac Antibody | OX-27 gallery image 2

Staining of BN rat spleen cells with Mouse anti Rat RT1Ac:FITC (MCA156F)

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MHC Class I RT1Ac Antibody | OX-27 gallery image 3

Staining of rat spleen lymphocytes with Mouse anti Rat RT1Ac (MCA156GA)

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MHC Class I RT1Ac Antibody | OX-27 gallery image 4

Published customer image:
Mouse anti Rat MHC Class I RT1Ac antibody, clone OX-27 used for the assessment of chimerism in a rat transplant model.
Image caption:
Peripheral blood multilineage chimerism in Group 5. Multilineage peripheral blood persisted for 12-13 weeks following vascularized hind-limb transplant, carried out by hind-limb transplant (HLT), at a low level in Group 5. At 13 weeks following vascularized hind-limb transplant, peripheral blood chimerism could hardly be detected by flow cytometry in 5 of 6 tolerant recipients in Group 5.

From: Quan Liu, Yong Wang, Atsunori Nakao, Wensheng Zhang, Vijay Gorantla, and Xin Xiao Zheng, “Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats,”
Clinical and Developmental Immunology, vol. 2013, Article ID 483856, 6 pages, 2013.

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MHC Class I RT1Ac Antibody | OX-27 gallery image 5

Published customer image:
Mouse anti Rat MHC class I RT1Ac antibody, clone OX-27 used for the evaluation of peripheral chimerism in a rat transplant model by flow cytometry.
Image caption:
Dynamic changes in peripheral and myeloid chimerism. (a) Time kinetics of chimerism in groups 5 and 6. At POD 7, they showed almost the same chimerism level (P>0.05). At POD 21, the level of chimerism in group 6 was mildly decreased, but it increased sharply in group 5 (P<0.05). At POD 42, chimerism in group 5 returned to a total level 8.7%±0.6% but it decreased to background level in group 6 (P<0.05). In addition, donor-origin CD45RA+ B cells in group 5 prevailed beginning at POD 21 and maintained thereafter. (b) Myeloid chimerism was detectable at 6.3±0.5% at POD 100 in group 5 and it never appeared in group 6 when allografts were rejected (n = 6, P<0.05).

From: Wang Y, Zheng Z, Wang Y, Liu J, Li N, et al. (2012) Role of Donor-Specific Regulatory T Cells in Long-Term Acceptance of Rat Hind Limb Allograft.
PLoS ONE 7(8): e43825.

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MHC Class I RT1Ac Antibody | OX-27 gallery image 6

Published customer image:
Mouse anti Rat MHC class I antibody, clone OX-27 used for the determination of levels of chimerism in a rat transplant model by flow cytometry and immunofluorescence
Image caption:
Flow cytometry analysis for myeloid chimerism and immunocytofluorescence staining for the presence of donor-origin cells in recipients' innate femurs. (a)The proportion of donor-origin cells observed from 2 representative recipients at POD 30 were at 5.7% and 5.5%, respectively, with the background level assessed at 0.1%. (b, c) The presence of RT1n cells in the contralateral femurs of recipients in group 5 and their absence in other groups was confirmed by immunocytofluorescence (×20).

From: Wang Y, Zheng Z, Wang Y, Liu J, Li N, et al. (2012) Role of Donor-Specific Regulatory T Cells in Long-Term Acceptance of Rat Hind Limb Allograft.
PLoS ONE 7(8): e43825.

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  • Mouse anti Rat MHC Class I RT1Ac:FITC
  • Mouse anti Rat MHC Class I RT1Ac
  • Mouse anti Rat MHC Class I RT1Ac:RPE
  • Mouse anti Rat MHC Class I RT1Ac
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    OX-27
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA156FFdatasheet pdfdatasheet pdf0.1 mg
    MCA156F
    MCA156GAC, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA156GA
    MCA156RC, Fdatasheet pdfdatasheet pdf0.25 mg
    MCA156R
    MCA156PEFdatasheet pdfdatasheet pdf100 Tests
    MCA156PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Rat MHC Class I RT1Ac antibody, clone OX-27 recognizes a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography on Protein A.
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A .
      Purified IgG prepared by affinity chromatography on Protein A
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography on Protein A.
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A .
    • Preservative Stabilisers
      0.09%Sodium Azide
      1.0%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1.0%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
    • Immunogen
      PHA activated rat lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      Isotype concentration 0.1 mg/ml
      Isotype concentration 1.0 mg/ml
      Isotype concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      This product should be stored undiluted.

      DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of reconstitution.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      1/100Pack Size: 0.1 mg
      1/50Pack Size: 0.25 mg
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      1/100Pack Size: 0.1 mg
      1/50Pack Size: 0.25 mg
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional MHC Class I RT1Ac Antibody Formats

    Formats Clone Applications Sizes available
    MHC Class I RT1Ac Antibody : RPE OX-27 F 100 Tests
    MHC Class I RT1Ac Antibody : Purified OX-27 C, F 0.1 mg | 0.25 mg
    MHC Class I RT1Ac Antibody : FITC OX-27 F 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      STAR9B
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      STAR117P
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      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
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      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:FITCMCA1210F0.1 mgF
        MCA1210F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA1210100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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