MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) Antibody

MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) Antibody gallery image 1

Western blot analysis of C6 cell lysate untreated or treated with anisomycin using Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) antibody

  • MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) Antibody thumbnail image 1
  • Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2693WBdatasheet pdfdatasheet pdf50 µl
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) antibody recognizes mitogen activated protein kinase (MAPK) 8 and 9, also known as c-Jun N-terminal kinases 1 and 2 or stress activated protein kinases 1 and 2, when phosphorylated on threonine 183 and tyrosine 185. This antibody also recognizes MAPK10, also known as c-Jun N-terminal kinase 3 or stress activated protein kinase 3, when phosphorylated on threonine 221 and tyrosine 223.

      As their name implies, MAPK8/9/10 are members of the serine/threonine MAP kinase family, which become activated by environmental stressors. In addition, activation through phosphorylation events has been reported upon stimulation of toll-like receptors and exposure to pro-inflammatory cytokines (Guma and Firestein 2012, Bogoyevitch and Kobe 2006).

      Two phosphorylation events are critical for the activation of the three MAPKs. The first is on threonine 183, or in the case of MAPK10 threonine 221, by dual specificity mitogen-activated kinase kinase 7 (MKK7) and the second is on tyrosine 185, or in the case of MAPK10 tyrosine 223, by MKK4 (Fleming 2000). The phosphorylation undertaken by MKK4 and MKK7 is counteracted by phosphatases of the MAP kinase phosphatase (MKP) family (Guma and Firestein 2012).

      The three MAPKs play a critical role in mediating cell signaling cascades by phosphorylating transcription factors, such as c-Jun and p53, thereby regulating cellular processes including proliferation and apoptosis (Guma and Firestein 2012).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) Antibody Formats

    Formats Applications Sizes available
    MAPK8/MAPK9 (pThr183/pTyr185)/MAPK10 (pThr221/pTyr223) Antibody : Purified WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

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          Recommended Positive Controls

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              Further Reading

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