Leukemia Inhibitory Factor | N/A

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  • Recombinant Rat Leukemia Inhibitory Factor
  • Recombinant Rat Leukemia Inhibitory Factor
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
  • Clone
    N/A
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    OBT1523GFNdatasheet pdfdatasheet pdf10 µg
    OBT1523G
    OBT1523FNdatasheet pdfdatasheet pdf5 µg
    OBT1523
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Rat Leukemia Inhibitory Factor is an Escherichia coli-derived recombinant protein corresponding to full-length mature rat leukemia inhibitory factor (LIF), a secreted peptide with the capability to induce terminal differentiation in leukemic cells.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Recombinant Protein - liquid
      Recombinant Protein - liquid
    • Reconstitution
    • Preparation
      Pack Size: 10 µgRecombinant rat LIF is expressed as a GST-fusion protein using a pGEX expression system, cleaved from GST via thrombin and purified by ion exchange chromatography.
      Pack Size: 5 µgRecombinant rat LIF is expresssed as a GST-fusion protein, cleaved from GST via thrombin and purified by ion exchange chromatography.
      Pack Size: 10 µgRecombinant rat LIF is expressed as a GST-fusion protein using a pGEX expression system, cleaved from GST via thrombin and purified by ion exchange chromatography.
      Pack Size: 5 µgRecombinant rat LIF is expresssed as a GST-fusion protein, cleaved from GST via thrombin and purified by ion exchange chromatography.
    • Preservative Stabilisers
      1mM DTT
      10% Glycerol
      0.02% Tween 20
      1mM DTT
      10% Glycerol
      0.02% Tween 20
    • Activity
      >1 x 108 units/mg.

      50 units is the amount required to induce differentiation in 50% of M1 colonies in 1ml agar cultures.
    • Purity
      >85% by HPLC and SDS-PAGE analyses.
      >85% by HPLC and SDS-PAGE analyses.
    • Approx. Protein Concentrations
      Pack Size: 10 µgTotal protein concentration 10ug/ml
      Pack Size: 5 µgTotal protein concentration 10.0ug/ml
      Pack Size: 10 µgTotal protein concentration 10ug/ml
      Pack Size: 5 µgTotal protein concentration 10.0ug/ml
    • Molecular Weight
      19.9 kDa (183 amino acid sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1ng/ug
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      organ regeneration
      retina development in camera-type eye
      positive regulation of adrenocorticotropin secretion
      negative regulation of angiogenesis
      growth factor activity
      immune response
      leukemia inhibitory factor receptor binding
      positive regulation of neuron projection development
      cytokine activity
      transdifferentiation
      extracellular space
      astrocyte differentiation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays1/10001/10000
    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays1/10001/10000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Leukemia Inhibitory Factor Formats

    Formats Clone Applications Sizes available
    Leukemia Inhibitory Factor : Rec. Protein N/A FN 5 µg | 10 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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