IL-4 antibody
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Rabbit anti Rat Interleukin-4
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-4
Antibody Quality and Performance Guarantee
| Rabbit anti Rat Interleukin-4 antibody specifically recognizes rat interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, a pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes. |
- Target Species
- Rat
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended - Antiserum Preparation
- Antisera to rat IL-4 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
- Carrier Free
- Yes
- Immunogen
- Recombinant rat IL-4.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 ug/ml | |
| Western Blotting | |
0.1ug/ml | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
- RRID
- AB_323569
- UniProt
- P20096
- Entrez Gene
- Il4
- GO Terms
- GO:0002674 negative regulation of acute inflammatory response
- GO:0002677 negative regulation of chronic inflammatory response
- GO:0005125 cytokine activity
- GO:0005136 interleukin-4 receptor binding
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007565 female pregnancy
- GO:0007584 response to nutrient
- View More GO Terms
- GO:0008083 growth factor activity
- GO:0042493 response to drug
- GO:0010155 regulation of proton transport
- GO:0014070 response to organic cyclic compound
- GO:0032733 positive regulation of interleukin-10 production
- GO:0034097 response to cytokine stimulus
- GO:0042113 B cell activation
- GO:0043193 positive regulation of gene-specific transcription
- GO:0045019 negative regulation of nitric oxide biosynthetic process
- GO:0045471 response to ethanol
- GO:0071288 cellular response to mercury ion
AAR16G
131015
156560
163062
164814
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with IL-4 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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