Rat IL-10 is a homodimeric protein, each 18.7 kDa subunit is 160 amino acid residues in length. IL-10 is a potent inhibitor of several cytokines including IFN gamma, IL-2 and TNF.
Interleukin-10 (IL-10) is produced primarily by activated T lymphocytes.
Purified recombinant protein - lyophilised
Reconstitute with 10ul water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage te addtion of 0.1% BSA is recommended,
Recombinant protein prepared from E.Coli
Determined by the dose-dependent inhibition of antigen-specific T-cell proliferation is ≤ 10ng/ml
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
Protein Molecular Weight
18.7kD (160 amino acid sequence)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
PRP29 may be used in ELISA applications with either AAR29 or AAR29B .
Histology Positive Control Tissue
PRP29 may be used under reducing or non-reducing western blotting conditions with either AAR29 or AAR29B .