IL-10 Antibody

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  • Rabbit anti Rat Interleukin-10
  • Rabbit anti Rat Interleukin-10:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAR29E, WBdatasheet pdfdatasheet pdf0.1 mg
    AAR29
    AAR29BE, WBdatasheet pdfdatasheet pdf50 µg
    AAR29B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Rat Interleukin-10 antibody recognizes rat interleukin-10 (IL-10)

      Rat IL-10 is a 18.7kD cytokine which exists as a homodimer.

      IL-10 is produced by a population of T-cells and is reported to inhibit the synthesis of several cytokines including IFN gamma, IL-2, IL-3 and TNF alpha.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG - lyophilised
      Purified IgG conjugated to Biotin - lyophilised
    • Reconstitution
      Reconstitute with 0.1 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
    • Antiserum Preparation
      Antisera to rat IL-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      None present
      None Present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml after reconstitution
      IgG concentration 0.1 mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      positive regulation of gene-specific transcription
      negative regulation of membrane protein ectodomain proteolysis
      response to lipopolysaccharide
      negative regulation of apoptosis
      cellular response to estradiol stimulus
      positive regulation of cytokine secretion
      negative regulation of tumor necrosis factor biosynthetic process
      inflammatory response
      extracellular space
      response to insulin stimulus
      positive regulation of transcription factor activity
      response to glucocorticoid stimulus
      response to carbon monoxide
      negative regulation of B cell proliferation
      negative regulation of cytokine secretion involved in immune response
      negative regulation of nitric oxide biosynthetic process
      receptor biosynthetic process
      negative regulation of interleukin-6 production
      immune response
      cytokine activity
      response to activity
      regulation of sensory perception of pain
      positive regulation of B cell apoptosis
      interleukin-10 receptor binding
      response to drug
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      AAR29 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with AAR29B as the detection antibody and PRP29 as the standard.
    • ELISA
      AAR29B may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with AAR29 as the capture antibody and PRP29 as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      AAR29 may be used under either reducing or non-reducing western blotting conditions with PRP29 as the positive control.
    • Western Blotting
      AAR29B may be used under either reducing or non-reducing western blotting conditions with PRP29 as the positive control.
    • Instructions For Use
    • Instructions For Use

    Additional IL-10 Antibody Formats

    Formats Applications Sizes available
    IL-10 Antibody : Biotin E, WB 50 µg
    IL-10 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Rat Interleukin-10AAR290.1 mgE, WB
          AAR29

          Recommended Positive Controls

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Recombinant Rat Interleukin-10PRP2910 µgE, FN, WB
            PRP29

            Histology Controls

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