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IgG2c antibody | MARG2c-5

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Mouse anti Rat IgG2c Heavy Chain:FITC

Product Type
Monoclonal Antibody
Clone
MARG2c-5
Isotype
IgG2a
Specificity
IgG2c
Region
HEAVY CHAIN

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Product Code Applications Pack Size List Price Your Price Qty
MCA1295F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.5 mg loader
List Price Your Price
loader

Mouse anti Rat IgG2c heavy chain antibody, clone MARG2c-5 recognizes the heavy chain of rat immunoglobulin gamma 2C as determined by dot blot analysis.

Target Species
Rat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
50% Glycerol
Immunogen
IgG2c (Rat strain LOU/c).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:FITC MCA1210F F 0.1 mg loader
>
List Price Your Price
loader
Description Mouse IgG2a Negative Control:FITC

References for IgG2c antibody

  1. Delriviere, L.D. et al. (1998) Basic anatomical and physiological differences between species should be considered when choosing combinations for use in models of hepatic xenotransplantation: an investigation of the guinea pig-to-rat combination.
    Transplantation. 66: 112-5.
  2. Lin, Y. et al. (1999) Accommodated xenografts survive in the presence of anti-donor antibodies and complement that precipitate rejection of naive xenografts.
    J Immunol. 163: 2850-7.
  3. Lin, Y. et al. (2000) Long-term survival of hamster hearts in presensitized rats.
    J Immunol. 164: 4883-92.
  4. Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
    Int Immunol. 30 (2): 53-67.

Further Reading

  1. van den Berg, T.K. et al. (2001) Monoclonal antibodies against rat leukocyte surface antigens.
    Immunol Rev. 184: 109-16.

RRID
AB_323212
UniProt
P20762
Entrez Gene
LOC362795
GO Terms
GO:0003823 antigen binding

MCA1295F

154652 164601

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