IgG2c Antibody | 9337

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IgG2c Antibody | 9337 gallery image 1

Staining of dog peripheral blood lymphocytes with Rat (IgG2c) anti dog CD11/CD18 (MCA1040G) followed by Human anti Rat IgG2c:FITC (HCA142F)

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IgG2c Antibody | 9337 gallery image 2

Detection of Rat IgG2c (PRP06) with Human anti Rat IgG2c (HCA142) followed by Mouse anti Strep-tag:HRP (MCA2489P)

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IgG2c Antibody | 9337 gallery image 3

Detection of Rat IgG2c (PRP06) with Human anti Rat IgG2c:HRP (HCA142P)

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  • IgG2c Antibody | 9337 thumbnail image 1
  • IgG2c Antibody | 9337 thumbnail image 2
  • IgG2c Antibody | 9337 thumbnail image 3
  • Human anti Rat IgG2c:FITC
  • Human anti Rat IgG2c
  • Human anti Rat IgG2c:HRP
  • Human anti Rat IgG2c
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    9337
  • Isotype
    HuCAL Fab bivalent
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA142FFdatasheet pdfdatasheet pdf0.1 mg
    HCA142F
    HCA142PEdatasheet pdfdatasheet pdf0.1 mg
    HCA142P
    HCA142E, Fdatasheet pdfdatasheet pdf0.1 mg
    HCA142
    HCA142AE, Fdatasheet pdfdatasheet pdf50 µg
    HCA142A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Rat IgG2c antibody, clone 9337 recognizes Rat IgG2c.

      No cross reactivity is seen with other rat immunoglobulin subclasses.

      For best results we recommend diluting this antibody in HiSpec buffer (product code BUF049) to reduce background staining.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is conjugated to Horseradish Peroxidase (HRP) - liquid
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Purified Rat monoclonal IgG2c.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of reconstitution.
      12 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/500
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/500
      Flow Cytometry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
      This product is suitable for use in indirect ELISA applications. This product may be used in an indirect ELISA together with MCA2489P as the detection reagent. Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • ELISA
      This product is suitable for use in direct ELISA applications.
    • ELISA
      This product is suitable for use in indirect ELISA applications. This product may be used in an indirect ELISA together with MCA2489P as the detection reagent.. Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG2c Antibody Formats

    Formats Clone Applications Sizes available
    IgG2c Antibody : FITC 9337 F 0.1 mg
    IgG2c Antibody : Purified 9337 E, F 0.1 mg | 50 µg
    IgG2c Antibody : HRP 9337 E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
      MCA2200A488
      Mouse anti c-Myc:Alexa Fluor® 647MCA2200A647100 Tests/1mlF *
      MCA2200A647
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti c-Myc:FITCMCA2200F0.1 mgF *
      MCA2200F
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti c-Myc:RPEMCA2200PE100 TestsWB
      MCA2200PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
      MCA2200A488
      Mouse anti c-Myc:Alexa Fluor® 647MCA2200A647100 Tests/1mlF *
      MCA2200A647
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti c-Myc:FITCMCA2200F0.1 mgF *
      MCA2200F
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti c-Myc:RPEMCA2200PE100 TestsWB
      MCA2200PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MSx2 Negative ControlHCA102A50 µgC, E, F, P, WB
        HCA102A
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MSx2 Negative ControlHCA1020.1 mgC, E, F, P, WB
        HCA102

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
          MCA2489P
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
          MCA2489P

          Recommended Positive Controls

            Histology Controls

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