Purified protein prepared by affinity chromatography from tissue culture supernatant
Single band by agarose electrophoresis and immunoelectrophoresis
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
2. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.J Immunol Methods. 71 (1): 9-16.
3. Argüelles, M.E. et al. (1999) Performance of thiophilic adsorption chromatography in the purification of rat IgG2b monoclonal antibodies from serum- and protein-free culture supernatants.Biomed Chromatogr. 13 (6): 379-81.