Rat IgG2a Negative Control Antibody

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  • Rat IgG2a Negative Control:Alexa Fluor® 488
  • Rat IgG2a Negative Control
  • Rat IgG2a Negative Control:Pacific Blue®
  • Rat IgG2a Negative Control:FITC
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Isotype controls savings up to 65%
  • Product Type
    Negative/Isotype Control
  • Isotype
    IgG2a
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1124RFdatasheet pdfdatasheet pdf0.1 mg
    MCA1124R
    MCA1124FTFdatasheet pdfdatasheet pdf0.1 mg
    MCA1124FT
    MCA1124A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1124A488
    MCA1124PBFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1124PB
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti DNP antibody reacts with Dinitrophenyl Hapten and is suitable for use as a negative control for the measurement of non-specific binding of Rat monoclonal antibodies of isotype IgG2a to human tissue.

      Test results show that MCA1124B is also suitable for use as a negative control with canine tissue.
    • Intended Use
    • Target Species
      Negative Control
    • Product Form
      Purified IgG conjugated to Alexa Fluor®488 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to pacific Blue® - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      50%Glycerol
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      50%Glycerol
      0.1% Sodium Azide (NaN3)
      50% Glycerol
    • Immunogen
      Dinitrophenyl hapten conjugated to ovalbumin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunised LOU/c rat were fused with cells of the rat IR983F myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry*
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry*
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry*
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry*

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *This antibody should be used at the same concentration as the test antibody.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Rat IgG2a Negative Control Antibody Formats

    Formats Applications Sizes available
    Rat IgG2a Negative Control Antibody : Alexa Fluor® 488 F 100 Tests/1ml
    Rat IgG2a Negative Control Antibody : Pacific Blue® F 100 Tests/1ml
    Rat IgG2a Negative Control Antibody : FITC F 0.1 mg
    Rat IgG2a Negative Control Antibody : Purified F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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