Rat IgG1 Negative Control Antibody

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  • Rat IgG1 Negative Control:Alexa Fluor® 700
  • Rat IgG1 Negative Control:Biotin
  • Rat IgG1 Negative Control:Alexa Fluor® 488
  • Rat IgG1 Negative Control:FITC
  • Rat IgG1 Negative Control:Low Endotoxin
  • Rat IgG1 Negative Control
  • Rat IgG1 Negative Control:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
    Rat IgG1 isotype control for measurement of non specific binding of rat monoclonal antibodies. This antibody recognizes a human surface antigen.
    Isotype controls savings up to 65%
    • Product Type
      Negative/Isotype Control
    • Isotype
      IgG1
    7 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA1211BFdatasheet pdfdatasheet pdf0.1 mg
      MCA1211B
      MCA1211ELC, E, Fdatasheet pdfdatasheet pdf0.5 mg
      MCA1211EL
      MCA1211C, E, Fdatasheet pdfdatasheet pdf1 ml
      MCA1211
      MCA1211FFdatasheet pdfdatasheet pdf100 Tests
      MCA1211F
      MCA1211A700Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA1211A700
      MCA1211A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA1211A488
      MCA1211A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA1211A647
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Rat IgG1 Negative Control antibody is suitable for the measurement of non-specific binding of rat monoclonal antibodies of isotype IgG1 to target cells. Rat IgG1 Negative Control antibody is intendend for use on mouse tissues.

        N.B. This Rat IgG1 Negative Control antibody recognizes a human cell surface marker, and therefore cannot be used as a negative control in this species.
      • Intended Use
      • Target Species
        Negative Control
      • Product Form
        Purified IgG conjugated to Alexa Fluor® 700 - liquid
        Purified IgG conjugated to Biotin - liquid
        Purified IgG conjugated to Alexa488 - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Alexa Fluor® 647 - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Preservative Stabilisers
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        None present
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.05 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from immunized PVG-RT1u rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
      • Storage
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at -20oC only.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
      • Acknowledgements
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*
      • Application NameYesNoMin DilutionMax Dilution
        ELISA
        Flow Cytometry*
        Immunohistology - Frozen
      • Application NameYesNoMin DilutionMax Dilution
        ELISA
        Flow Cytometry*
        Immunohistology - Frozen
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry*

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

        * It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 106 cells in 100ul.
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                References

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