IgG1 Antibody | 8941

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IgG1 Antibody | 8941 gallery image 1

Rat IgG1 detected with Human anti Rat IgG1:HRP (HCA118P)

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IgG1 Antibody | 8941 gallery image 2

Rat IgG1 detected with Human anti Rat IgG1 (HCA118)

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  • IgG1 Antibody | 8941 thumbnail image 1
  • IgG1 Antibody | 8941 thumbnail image 2
  • Human anti Rat IgG1
  • Human anti Rat IgG1:HRP
  • Human anti Rat IgG1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    8941
  • Isotype
    HuCAL Fab bivalent
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA118PEdatasheet pdfdatasheet pdf0.1 mg
    HCA118P
    HCA118Edatasheet pdfdatasheet pdf0.1 mg
    HCA118
    HCA118AEdatasheet pdfdatasheet pdf50 µg
    HCA118A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Rat IgG1 antibody, clone 8941 recognizes Rat IgG1

      No cross reactivity is seen with other rat immunoglobulin subclasses.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Pack Size: 0.1 mgA bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      Pack Size: 50 µgA bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to horseradish peroxidase (HRP) - liquid.
      Pack Size: 0.1 mgA bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
      Pack Size: 50 µgA bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a Strep-tag at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
    • Preparation
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
      StrepTactin affinity chromatography
    • Preservative Stabilisers
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      Pack Size: 50 µg0.01% Thiomersal
      0.01% Thiomersal
      1% Bovine Serum Albumin
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      Pack Size: 50 µg0.01% Thiomersal
    • Immunogen
      Purified Rat monoclonal IgG1 kappa
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.1 mgTotal protein concentration 0.1mg/ml
      Pack Size: 50 µgAntibody concentration 0.5 mg/ml
      Total protein concentration 0.1mg/ml
      Pack Size: 0.1 mgTotal protein concentration 0.1mg/ml
      Pack Size: 50 µgAntibody concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 0.1 mgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/501/500
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
      This product is suitable for use in direct ELISA applications.
    • ELISA
      This product is suitable for use in direct ELISA applications. Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG1 Antibody Formats

    Formats Clone Applications Sizes available
    IgG1 Antibody : Purified 8941 E 0.1 mg | 50 µg
    IgG1 Antibody : HRP 8941 E 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
      MCA2489P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
      MCA2489P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:Alk.Phos.MCA2200A0.1 mgC, E, P, WB*
      MCA2200A
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
      MCA2489P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

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