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IgG antibody

Rabbit F(ab')2 anti Rat IgG:DyLight®550

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

Product Code Applications Pack Size List Price Your Price Qty
STAR16D550
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF WB 0.1 mg loader
List Price Your Price
loader

Rabbit F(ab')2 anti Rat IgG antibody recognizes rat IgG, detecting all subclasses in immunodiffusion assays.

Target Species
Rat
Product Form
Purified IgG conjugated to DyLight®550 - liquid
Antiserum Preparation
Antisera to rat IgG were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified polyclonal rat IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Dylight®550 562 576
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/400
Immunofluorescence 1/100 1/400
Western Blotting 1/10,000 1/50,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul

UniProt
P20759
P20762
P20761
P20760
Entrez Gene
Ighg
LOC679045
LOC362795
GO Terms
GO:0003823 antigen binding

STAR16D550

159097

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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