IgG Antibody

//www.bio-rad-antibodies.com/rat-igg-antibody-643001.html?RenderPageSpecific=1
IgG Antibody gallery image 1

Published customer image:
FITC conjugated Donkey anti Rat IgG antibody used to detect bound Rat anti Mouse CD31 antibody in a choroidal neovascularization study using immunofluorescence microscopy.
Image caption:
Choroidal neovascularization (CNV) detection by CD31 immunofluorescence. Mice that received only the vehicle (upper left, group V) had larger areas of CNV than mice that received the supplement (upper right) and/or anti-vascular endothelial growth factor (VEGF) treatment. Treatment groups included the nutritional supplement (S) group, the supplement and anti-vascular endothelial growth factor agent (S + aVEGF) group, and the vehicle and anti-VEGF agent (V + aVEGF) group.

From: Ivanescu AA, Fernández-Robredo P, Heras-Mulero H, Sádaba-Echarri LM, García-García L, Fernández-García V, Moreno-Orduna M, Redondo-Exposito A, Recalde S, García-Layana A. Modifying Choroidal Neovascularization Development with a Nutritional Supplement in Mice.
Nutrients. 2015 Jul 6;7(7):5423-42.

Enlarge
  • IgG Antibody thumbnail image 1
  • Donkey anti Rat IgG (H/L):RPE (Mouse Adsorbed)
  • Donkey anti Rat IgG (H/L):HRP (Mouse Adsorbed)
  • Donkey anti Rat IgG (H/L):FITC (Mouse Adsorbed)
  • Donkey anti Rat IgG (H/L) (Mouse Adsorbed)
  • Donkey anti Rat IgG (H/L):Alk. Phos. (Mouse Adsorbed)
  • Donkey anti Rat IgG (H/L):Biotin (Mouse Adsorbed)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    643009Fdatasheet pdfdatasheet pdf0.5 mg
    643009
    643002IFdatasheet pdfdatasheet pdf1 mg
    643002
    643001C, Edatasheet pdfdatasheet pdf1 mg
    643001
    643008C, Edatasheet pdfdatasheet pdf1 mg
    643008
    643005C, Edatasheet pdfdatasheet pdf1 ml
    643005
    643004C, E, WBdatasheet pdfdatasheet pdf1 ml
    643004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Donkey anti Rat IgG antibody recognizes rat IgG heavy and light chains and demonstrates minimal cross reactivity with mouse serum proteins.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Ig Fraction conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Ig Fraction - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Rat IgG covalently linked to agarose.
    • Preservative Stabilisers
      0.09% Sodium azide
      Stabilising agent
      50% Glycerol
      0.1% Sodium Azide (NaN3)
      None present
      0.1%Sodium Azide (NaN3)
      50%Glycerol
      0.1% Sodium azide
    • Immunogen
      Rat IgG.
    • Purity
    • Approx. Protein Concentrations
      0.5 mg/ml
      1.0 mg/ml
      1.0 mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Borate buffered saline
      TRIS buffered saline, 1mM MgCl2
      Phosphate buffered saline
    • Storage
      +4ºC DO NOT FREEZE
      This product is photosensitive and should be protected from light.
      -20ºC only (ship +4ºC)
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      -20ºC only (ship +4ºC)
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      Please see label for expiry date.
      18 months from the date of despatch.
      Please see label for expiry date.
      Please see label for expiry date.
      Please see label for expiry date.
      18 months from the date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/4000
      Immunohistology - Frozen
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : Biotin C, E 1 mg
    IgG Antibody : FITC IF 1 mg
    IgG Antibody : RPE F 0.5 mg
    IgG Antibody : Alk. Phos. C, E, WB 1 ml
    IgG Antibody : Purified C, E 1 mg
    IgG Antibody : HRP C, E 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...