IgG Antibody

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  • Rabbit F(ab')2 anti Rat IgG:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    613005C, E, IBdatasheet pdfdatasheet pdf1 ml
    613005
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • 613005 recognises the heavy and light chains of rat IgG1, IgG2a, IgG2b and IgG2c. This product also reacts with the light chains of rat IgM as demonstrated by ELISA.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      F(ab')2 fragment of IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to rat IgG was raised by repeated immunisations of rabbits with rat IgG paraproteins. The preparation was purified by affinity chromatography on rat IgG covalently linked to agarose, and gel filtration chromatography of pepsin digested antibody.
    • Preservative Stabilisers
      50% Glycerol
    • Immunogen
      Rat IgG
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend
    • Shelf Life
      18 months from the date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/40001/8000
      Immunoblotting1/10001/2000
      Immunohistology - Frozen1/501/200

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : HRP C, E, IB 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

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