5204-2304 is specific for the heavy and light chains of rat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, rat IgG and rat serum.
Purified IgG conjugated to Biotin - lyophilised
Reconstitute using 1 ml of sterile distilled water
Purified IgG prepared by immunoaffinity chromatography using Rat IgG coupled to agarose
Sodium Azide (NaN3)
Bovine Serum Albumin
Rat IgG, whole molecule
Approx. Protein Concentrations
Ig concentration 2.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. After reconstitution store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.