IgG Antibody

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IgG Antibody gallery image 1

Staining of mouse spleen cryosection with Rat anti Mouse CD68 (MCA1957) followed by Goat anti Rat IgG:HRP (305005) showing staining of macrophages in the red pulp

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  • IgG Antibody thumbnail image 1
  • Goat anti Rat IgG (H/L):RPE
  • Goat anti Rat IgG (H/L):Tritc
  • Goat anti Rat IgG (H/L):HRP
  • Goat anti Rat IgG (H/L):FITC
  • Goat anti Rat IgG (H/L)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    305009Fdatasheet pdfdatasheet pdf0.5 mg
    305009
    305003F, IFdatasheet pdfdatasheet pdf1 mg
    305003
    305002F, IFdatasheet pdfdatasheet pdf1 mg
    305002
    305001E, IPdatasheet pdfdatasheet pdf1 mg
    305001
    305005C, E, IBdatasheet pdfdatasheet pdf1 ml
    305005
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Rat IgG antibody reacts with the heavy and light chains of rat IgG, and the light chains of rat IgA and IgM. Cross reactivity with mouse immunoglobulins has been minimised by cross adsorption with pooled mouse sera and plasmacytoma/hybridoma proteins.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Ig Fraction conjugated to R. Phycoerythrin (RPE) - liquid
      Ig Fraction conjugated to Rhodamine (TRITC) - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid.
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Ig Fraction - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium azide
      Stabilising agent
      0.09% Sodium azide
      50% Glycerol
      0.1% Sodium azide
      None present
    • Immunogen
      Rat IgG
    • Purity
    • Approx. Protein Concentrations
      0.5 mg/ml
      1.0 mg/ml
      1.0 mg/ml
      1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Borate buffered saline
    • Storage
      +4ºC DO NOT FREEZE
      This product is photosensitive and should be protected from light.
      +4ºC, -20ºC if preferred
      This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      -20ºC only (ship +4ºC)
    • Shelf Life
      Please see label for expiry date.
      Please see label for expiry date.
      12 months from date of despatch.
      18 months from the date of despatch.
      18 months from the date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/40001/8000
      Immunoblotting
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoprecipitation

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : Tritc F, IF 1 mg
    IgG Antibody : Purified E, IP 1 mg
    IgG Antibody : FITC F, IF 1 mg
    IgG Antibody : HRP C, E, IB 1 ml
    IgG Antibody : RPE F 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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