Granulocytes Antibody | HIS48

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Granulocytes Antibody | HIS48 gallery image 1

Published customer image:
Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 used for stauiining granulocytes in rat corneal sections by immunofluorescence.
Image caption:
Immunofluorescence images on flat-mounted corneas in a model of endotoxin-induced uveitis analyzed with a 3D laser confocal microscope with a 20X enlargement. Immunostainings with anti-CD68 for macrophages (A), anti-TCR alpha/beta for lymphocytes (B), anti-MCA967 for granulocytes (C), and IgG1 mouse antibody for isotypic control (D) were revealed with secondary antibody, Alexa Fluor (in green) while the nuclear chromatin was stained with propidium iodide (in red). A double immunostaining with Alexa Fluor 488 phalloidin (in green), and antibody to CD68 (in red) was performed to localize the macrophages among the corneal layers, notably on the endothelium (E).

From: Trinh L, Brignole-Baudouin F, Labbé A, Raphaël M, Bourges JL, Baudouin C. The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
Mol Vis. 2008 Jun16;14:1149-56.

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  • Mouse anti Rat Granulocytes and Erythroid Cells
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    HIS48
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA967C *, F, IF, P *datasheet pdfdatasheet pdf2 ml
    MCA967
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 recognizses granulocytes and erythroid cells.

      Clone HIS48 has frequently been used to stain rat neutrophils in immunohistochemistry (Reckless et al. 2001).
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
      Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 8% foetal calf serum
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      PVG rat spleen cell suspension.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only

    Additional Granulocytes Antibody Formats

    Formats Clone Applications Sizes available
    Granulocytes Antibody : S/N HIS48 C *, F, IF, P * 2 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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