Granulocytes Antibody | HIS48

Granulocytes Antibody | HIS48 gallery image 1

Published customer image:
Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 used for stauiining granulocytes in rat corneal sections by immunofluorescence.
Image caption:
Immunofluorescence images on flat-mounted corneas in a model of endotoxin-induced uveitis analyzed with a 3D laser confocal microscope with a 20X enlargement. Immunostainings with anti-CD68 for macrophages (A), anti-TCR alpha/beta for lymphocytes (B), anti-MCA967 for granulocytes (C), and IgG1 mouse antibody for isotypic control (D) were revealed with secondary antibody, Alexa Fluor (in green) while the nuclear chromatin was stained with propidium iodide (in red). A double immunostaining with Alexa Fluor 488 phalloidin (in green), and antibody to CD68 (in red) was performed to localize the macrophages among the corneal layers, notably on the endothelium (E).

From: Trinh L, Brignole-Baudouin F, Labbé A, Raphaël M, Bourges JL, Baudouin C. The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
Mol Vis. 2008 Jun16;14:1149-56.

  • Granulocytes Antibody | HIS48 thumbnail image 1
  • Mouse anti Rat Granulocytes and Erythroid Cells
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA967C*, F, IF, P*datasheet pdfdatasheet pdf2 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Rat granulocytes and erythroid cells antibody, clone HIS48 recognizses granulocytes and erythroid cells.

      Clone HIS48 has frequently been used to stain rat neutrophils in immunohistochemistry (Reckless et al. 2001).
    • Intended Use
    • Target Species
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
      Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 8% foetal calf serum
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      PVG rat spleen cell suspension.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only

    Additional Granulocytes Antibody Formats

    Formats Clone Applications Sizes available
    Granulocytes Antibody : S/N HIS48 C*, F, IF, P* 2 ml
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138A1 mlC, E, P, WB
      Human anti Mouse IgM:FITCHCA040F0.1 mgF
      Human anti Mouse IgM:HRPHCA040P0.1 mgE
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Frozen
                Immunohistology - Paraffin


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