Glucose Transporter 4 Antibody | 1F8

//www.bio-rad-antibodies.com/rat-glucose-transporter-4-antibody-1f8-4670-1725.html?RenderPageSpecific=1
Glucose Transporter 4 Antibody | 1F8 gallery image 1

3T3-L1 lysate probed with Mouse anti Rat GLUT4:HRP (4670-1725P)

Enlarge
Glucose Transporter 4 Antibody | 1F8 gallery image 2

HeLa cells stained with Mouse anti Glucose Transporter 4:FITC (4670-1725F) and counterstained with DAPI

Enlarge
Glucose Transporter 4 Antibody | 1F8 gallery image 3

Published customer image:
IMouse anti Rat glucose transporter-4 antibody, clone 1F8 used for the evaluation of GLUT-4 expression in myotube lysates by western blotting.
Image caption:
Effect of resveratrol on translocation of GLUT4 to plasma membrane and phosphorylation of AMPK and Akt. A: Translocation of GLUT4 to plasma membrane of L6 myotubes which were exposed to 100 µM resveratrol for 15 min. GLUT4 and plasma membrane were detected by western blotting analyses with anti-GLUT4 and anti-Na+/K+ ATPase antibodies. Post-PM and PM indicate post plasma membrane and plasma membrane fractions, respectively. B, C: Effect of resveratrol on phosphorylation of AMPK (B) and Akt (C). L6 myotubes were exposed to resveratrol (10 µM) for indicated time intervals. Western blotting analyses were carried out with anti-AMPK, anti-phospho-AMPK, anti-Akt and anti-phospho-Akt antibodies. D, E: Time-dependent changes of GLUT4 translocation in L6 myotubes at 10 μM (D) or 100 μM (E) resveratrol. L6 myotubes were exposed to resveratrol for indicated time intervals. Plasma membrane fractions of L6 myotubes were subjected to western blotting analyses.

From: Minakawa M, Kawano A, Miura Y, Yagasaki K. Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic β-cells. J Clin Biochem Nutr. 2011 May;48(3):237-44.

Enlarge
  • Glucose Transporter 4 Antibody | 1F8 thumbnail image 1
  • Glucose Transporter 4 Antibody | 1F8 thumbnail image 2
  • Glucose Transporter 4 Antibody | 1F8 thumbnail image 3
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1F8
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4670-1725PC, WBdatasheet pdfdatasheet pdf0.1 mg
    4670-1725P
    4670-1725FIFdatasheet pdfdatasheet pdf0.1 mg
    4670-1725F
    4670-1725GAC, IF, IP, P, WBdatasheet pdfdatasheet pdf0.1 mg
    4670-1725GA
    4670-1725PTC, WBdatasheet pdfdatasheet pdf25 µg
    4670-1725PT
    4670-1725C, IF, IP, P *, WBdatasheet pdfdatasheet pdf50 µg
    4670-1725
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti glucose transporter 4 antibody, clone 1F8 originally raised against rat intracellular low density microsomes (James et al. 1987) recognizes an epitope in the cytoplasmic region of Glucose transporter 4 (GLUT4), an insulin-regulated facilitative glucose transporter found in adipose tissue and striated muscle. When stimulated by insulin, GLUT4 translocates from intracellular stores to the cell surface, facilitating passive diffusion of circulating glucose into muscle and fat cells. GLUT4 is also stimulated to locate to the cell surface by muscle contraction, particularly in cardiac muscle (James et al. 1988).

      Mouse anti glucose transporter 4 antibody, clone 1F8 has been used successfully to demonstrate the localization of GLUT4 to the basolateral side of ductal structures in the rat submandibular salivary gland in formalin fixed, paraffin embedded material (Cetik et al. 2014).
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Monkeyyes
      Dogno
      Rabbityes
      Humanyes
      Pigyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
    • Preservative Stabilisers
      0.01% Thiomersal

      HRP Stabiliser (BUF052A)
      Pack Size: 50 µg
      0.09%Sodium Azide (NaN3)
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
      0.01% Thiomersal

      HRP Stabiliser (BUF052A)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      Pack Size: 50 µg
      0.09%Sodium Azide (NaN3)
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
    • Immunogen
      Partially purified vesicles containing insulin-responsive glucose transporter 4.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      Pack Size: 50 µgIgG concentration 1 mg/ml
      Pack Size: 0.1 mgIgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.1mg/ml
      Pack Size: 50 µgIgG concentration 1 mg/ml
      Pack Size: 0.1 mgIgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      brown fat cell differentiation
      insulin-responsive hydrogen:glucose symporter activity
      coated pit
      soluble fraction
      external side of plasma membrane
      cytoplasmic vesicle membrane
      protein binding
      response to ethanol
      integral to plasma membrane
      D-glucose transmembrane transporter activity
      carbohydrate metabolic process
      microsome
      multivesicular body
      trans-Golgi network transport vesicle
      glucose import
      perinuclear region of cytoplasm
      insulin-responsive compartment
      integral to membrane of membrane fraction
      extracellular vesicular exosome
      vesicle membrane
      glucose homeostasis
      sarcolemma
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting1/1001/1000
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting1/1001/1000
    • Application NameYesNoMin DilutionMax Dilution
      ImmunofluorescenceNeat1/25
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Glucose Transporter 4 Antibody Formats

    Formats Clone Applications Sizes available
    Glucose Transporter 4 Antibody : HRP 1F8 C, WB 25 µg | 0.1 mg
    Glucose Transporter 4 Antibody : Purified 1F8 C, IF, IP, P, P *, WB 0.1 mg | 50 µg
    Glucose Transporter 4 Antibody : FITC 1F8 IF 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

              Write your review

              You may also be interested in...