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GAP43 (pSer41) Antibody

GAP43 (pSer41) Antibody gallery image 1

Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~50 kDa GAP-43 protein phosphorylated at Ser41

  • GAP43 (pSer41) Antibody thumbnail image 1
  • Rabbit anti GAP43 (pSer41)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP902WB*datasheet pdfdatasheet pdf0.1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti phosphorylated GAP43 antibody recognizes neuromodulin, also known as growth-associated Protein 43, GAP-43, Axonal membrane protein GAP-43, Neural phosphoprotein B-50 or pp46. Rabbit anti phosphorylated GAP43 antibody recognizes GAP43 only when phosphorylated at serine 41.

      GAP-43 is expressed in neuronal growth cones during development and during axonal regeneration and is phosphorylated at Ser41 by protein kinase C.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      HumanExpected from Sequence
      BovineExpected from Sequence
      ChickenExpected from Sequence
      DogExpected from Sequence
      MonkeyExpected from Sequence
      XenopusExpected from Sequence
      ZebrafishExpected from Sequence
      MouseExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to phosphorylated GAP-43 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.01%Bovine Serum Albumin
    • Immunogen
      Synthetic phosphopeptide corresponding to an amino acid sequence within GAP-43 which includes phosphorylated Ser41.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM HEPES pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      response to wounding
      growth cone membrane
      activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway
      regulation of growth
      cell junction
      cell differentiation
      calmodulin binding
      nervous system development
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP902 detects a band of approximately 50kDa in rat cortex lysates.
    • Instructions For Use

    Additional GAP43 (pSer41) Antibody Formats

    Formats Applications Sizes available
    GAP43 (pSer41) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • GAP43 (pSer41) Antibody thumbnail image 1

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