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Erk/MAPK (pThr202/pTyr204) Antibody

Erk/MAPK (pThr202/pTyr204) Antibody gallery image 1

Western blot of human T47D cells showing phospho-specific immunolabeling of the ~42-44 kDa ERK/MAPK protein doublet

  • Erk/MAPK (pThr202/pTyr204) Antibody thumbnail image 1
  • Rabbit anti Erk/MAPK (pThr202/pTyr204)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP901WB*datasheet pdfdatasheet pdf0.1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti phosphorylated ERK/MAPK antibody recognizes the ~42-44 kDa extracellular-signal regulated kinase/mitogen activated protein kinase (ERK/MAPK), when phosphorylated at threonine 202 and tyrosine 204.

      ERK/MAPK is an important integration point of many signalling pathways, for a range of cellular processes including proliferation, differentiation, transcription regulation and development. Upon phosphorylation, activated ERK/MAPK translocates to the nucleus, where it phosphorylates nuclear targets.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP901 recognises a band of approximately 43kD in human T47D lysates.
    • Instructions For Use

    Additional Erk/MAPK (pThr202/pTyr204) Antibody Formats

    Formats Applications Sizes available
    Erk/MAPK (pThr202/pTyr204) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Erk/MAPK (pThr202/pTyr204) Antibody thumbnail image 1

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