ELK-1 (pSer383) Antibody

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ELK-1 (pSer383) Antibody gallery image 1

Western blot of non-phospho- and phospho- recombinant Elk-1 showing specific immunolabeling of the ~46 kDa Elk-1 protein phosphorylated at Ser383

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  • ELK-1 (pSer383) Antibody thumbnail image 1
  • Rabbit anti ELK-1 (pSer383)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP900WB*datasheet pdfdatasheet pdf0.1 ml
    AHP900
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti phosphorylated ELK-1 antibody recognizes rat ETS domain-containing protein Elk-1 (ELK-1) when phosphorylated at serine 383.

      ELK-1 is a member of the ternary complex factor (TCF) family of transcription factors, which is thought to play a role in mediating gene expression in response to growth factors.

      ELK-1 can be phosphorylated at serine 383 and 389 in its C-terminal region in response to the activation of extracellular signal-regulated kinase (ERK) proteins. Phosphorylation of ELK-1 results in an increase in its transcriptional activity, leading to the transcription of growth-related proteins, such as c-Fos.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      HumanExpected from Sequence
      MonkeyExpected from Sequence
      MouseExpected from Sequence
      DogExpected from Sequence
      ZebrafishExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to phosphorylated ELK-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.01%Bovine Serum Albumin
      50%Glycerol
    • Immunogen
      Synthetic phosphopeptide corresponding to an amino acid sequence within ELK-1 which includes phosphorylated Ser383.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM HEPES pH7.5
      150mM NaCl
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      Toll signaling pathway
      MyD88-dependent toll-like receptor signaling pathway
      MyD88-independent toll-like receptor signaling pathway
      toll-like receptor 2 signaling pathway
      toll-like receptor 3 signaling pathway
      nerve growth factor receptor signaling pathway
      nucleus
      transcription activator activity
      stress-activated MAPK cascade
      sequence-specific DNA binding
      positive regulation of transcription from RNA polymerase II promoter
      sequence-specific DNA binding transcription factor activity
      protein binding
      innate immune response
      toll-like receptor 1 signaling pathway
      toll-like receptor 4 signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      (1)
      For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP900 detects a band of approximately 46kDa in Western blots.
    • Instructions For Use

    Additional ELK-1 (pSer383) Antibody Formats

    Formats Applications Sizes available
    ELK-1 (pSer383) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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