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Darpp-32 (pThr34) Antibody

Darpp-32 (pThr34) Antibody gallery image 1

Western blot of rat caudate showing phosphospecific immunolabeling of the ~32 kDa DARPP-32 protein phosphorylated at Thr34

  • Darpp-32 (pThr34) Antibody thumbnail image 1
  • Rabbit anti Darpp-32 (pThr34)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP897WB*datasheet pdfdatasheet pdf0.1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Rat DARP-32 (pThr34) antibody recognizes rat DARPP-32, when phosphorylated at threonine 34. DARPP-32 is also known as Protein phosphatase 1 regulatory subunit 1B or Dopamine- and cAMP-regulated neuronal phosphoprotein. DARPP-32 is a 205 amino acid ~32 kDa member of the protein phosphatase inhibitor 1 family.

      DARPP-32 is principally expressed in striatal medium spiny neurons, and plays a critical role in the regulation of dopaminergic neurotransmission.

      DARPP-32 can act either as a phosphatase inhibitor or as a kinase inhibitor, depending on its relative state of phosphorylation . Phosphorylation at threonine 34 converts DARPP-32 into an inhibitor of protein phosphatase-1 (PP-1) whilst phosphorylation at threonine 75 switches the protein to an inhibitor of protein kinase A (PKA) .

      G-protein coupled receptor 6 deficiency in a mouse model of Parkinsons disease leads to an increase in DARPP-32 (pThr34) in striatopalladial neurons with a concommitent increase in locomotor activity and reduced abnormal movements in the mouse dyskinesia model of Parkinson's disease, thus suggesting treatment other than dopamine replacement for the condition (Oekl et al. 2014).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      DogExpected from Sequence
      HumanExpected from Sequence
      BovineExpected from Sequence
      ChickenExpected from Sequence
      MonkeyExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to phosphorylated rat DARPP-32 were raised by repeated immunisations of rabbits with highly purified antigen.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.1%Bovine Serum Albumin
    • Immunogen
      Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr34.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM HEPES pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      receptor binding
      intracellular signal transduction
      protein phosphatase inhibitor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      For the detection of phosphoproteins, threonine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP897 detects a band of approximately 32kDa in rat caudate lysates.
    • Instructions For Use

    Additional Darpp-32 (pThr34) Antibody Formats

    Formats Applications Sizes available
    Darpp-32 (pThr34) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Darpp-32 (pThr34) Antibody thumbnail image 1
              • Application NameReference Images
                Western Blotting


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