Darpp-32 Antibody

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Western blot of the rat hippocampal lysate showing specific immunolabeling of the ~32 kDa DARPP protein

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  • Rabbit anti Darpp-32 (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1158WBdatasheet pdfdatasheet pdf0.1 ml
    AHP1158
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Rat DARPP-32 antibody recognizes rat Protein phosphatase 1 regulatory subunit 1B, also known as DARPP-32 or Dopamine- and cAMP-regulated neuronal phosphoprotein. DARPP-32 is a 205 amino acid ~32 kDa member of the protein phosphatase inhibitor 1 family. Originally discovered as a substrate for cAMP-dependent protein kinase which modulates cell signalling in neurons. DARPP-32 is converted into an inhibitor of protein phosphatase-1 when phosphorylated by protein kinase A at threonine 34, as a result of dopaminergic receptor stimulation (Bibb et al. 1999). However this phosphorylation is reversed on stimulation of N-methyl-D-aspartate (NMDA) receptors by glutamate (Halpain et al. 1990). Conversely when phosphorylated at threonine-75 by CDK5, DARPP-32 becomes an inhibitor of protein kinase A (Bibb et al. 1999).

      DARPP-32 is highly expressed in striatonigral and striatopallidal projection neurons but is not observed in cholinergic and neuropeptide Y-containing striatal interneurons (Anderson and Reiner 1991). A truncated form of DARPP-32, t-DARPP is over-expressed in gastric cancer cells (El-Rifai et al. 2002) and, further t-DARPP has subsequently been identified as overexpressed in Her-2 positive breast cancer cells and implicated in resistance of these cells to Herceptin treament (Gu et al. 2009).

      DARPP-32 appears to have evolved early in vertebrate evolution and may have first appeared in gnathostomes. The immunogen sequence for Rabbit anti rat DARPP-32 antibody is identical in canine, chicken, human, mouse and Xenopus, therefore the antibody therefore is expected to recognize DARP-32 in all these species. Rabbit anti rat DARPP-32 antibody is not expected to recognize t-DARPP as this lacks the amino sequence used for immunization. For the identification of specific phospho DARPP-32 forms the use af antibodies against the threonine 34 and 75 phospho-epitopes is recommended.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      ZebrafishExpected from Sequence
      MouseExpected from Sequence
      DogExpected from Sequence
      XenopusExpected from Sequence
      BovineExpected from Sequence
      ChickenExpected from Sequence
      HumanExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to rat DARPP-32 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.01%Bovine Serum Albumin
      50%Glycerol
    • Immunogen
      Peptide corresponding to the N-terminal region of rat DARPP-32, conjugated to keyhole limpet haemocyanin (KLH).
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM Hepes pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      intracellular signal transduction
      receptor binding
      cytoplasm
      protein phosphatase inhibitor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1158 detects a band of approximately 32kDa in rat hippolampal lysate.
    • Instructions For Use

    Additional Darpp-32 Antibody Formats

    Formats Applications Sizes available
    Darpp-32 Antibody : Purified WB 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
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          Recommended Positive Controls

            Histology Controls

              References

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