CREB (pSer133) Antibody

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CREB (pSer133) Antibody gallery image 1

Western blot of a forskolin-stimulated rat hippocampal slice showing phosphospecific immunolabeling of the ~45 kDa CREB protein phosphphorylated at Ser133

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  • CREB (pSer133) Antibody thumbnail image 1
  • Rabbit anti CREB1 (pSer133)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP896WB*datasheet pdfdatasheet pdf0.1 ml
    AHP896
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti phosphorylated CREB antibody recognizes rat cyclic AMP response element binding protein 1, also known as CREB or CREB-1, when phosphorylated at serine 133.

      CREB is a member of the leucine zipper family of transcription factors, which is involved in a wide range of intracellular signalling processes.

      Phosphorylation of serine 133 has been demonstrated by several protein kinases, including protein kinase A (PKA), mitogen activated protein kinase (MAPK) and Ca2+ /calmodulin-dependent protein kinase (CaMK). This phophorylation is required for CREB mediated transcription.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      DogExpected from Sequence
      XenopusExpected from Sequence
      ZebrafishExpected from Sequence
      HumanExpected from Sequence
      BovineExpected from Sequence
      MonkeyExpected from Sequence
      ChickenExpected from Sequence
      SheepExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to phosphorylated rat CREB1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.01%Bovine Serum Albumin
      50%Glycerol
    • Immunogen
      Synthetic phosphopeptide corresponding to an amino acid sequence within CREB which includes phosphorylated Ser133.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      10mM HEPES pH7.5
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
      protein dimerization activity
      specific RNA polymerase II transcription factor activity
      sequence-specific DNA binding
      regulation of apoptosis
      double-stranded DNA binding
      transforming growth factor beta receptor signaling pathway
      sequence-specific enhancer binding RNA polymerase II transcription factor activity
      regulation of circadian rhythm
      positive regulation of gene-specific transcription from RNA polymerase II promoter
      transcription factor complex
      nuclear chromatin
      transcription factor binding
      positive regulation of transforming growth factor-beta3 production
      mitochondrion
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      (1)
      For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP896 detects a band of approximately 45kDa in rat hippocampus lysates that had been incubated in the presence of forskolin.
    • Instructions For Use

    Additional CREB (pSer133) Antibody Formats

    Formats Applications Sizes available
    CREB (pSer133) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting

              References

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