2080-0157 is a recombinant protein corresponding to rat Choline Acetyltransferase (ChAT), an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
This product may be used as blocking peptide for our human choline acetyltransferase antibody (product 2080-0000) at a suggested concentration range of 2-5 µg/ml. The antigen should be incubated with the antibody for 3 hours at 4ºC before being used on tissue sections.
Purified recombinant ChAT- liquid
Purified recombinant rat choline acetyltransferase expressed in Escherichia coli.
Approx. Protein Concentrations
Total protein concentration 0.25 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.