2080-0101 is specific for choline acetyltransferase and stains cholinergic neurons in the brain and central nervous system.Choline acetyltransferase is an enzyme, present the presynaptic ends of axons, that catalyzes the transfer of the acetyl group of acetyl CoA to choline, forming the neurotransmitter acetylcholine.
N.B. Antibody reactivity and working conditions may vary between species.
Choline acetyltransferase purified from rat brain
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
4. Matthews, D. A. et al. (1987) An immunocytochemical study of choline acetyltransferase-containing neurons and axon terminals in normal and partially deafferented hippocampal formationBrain Res. 402: 30 - 43
5. Hagg, T. et al. (1989) Nerve growth factor (NGF) reverses axotomy-induced decreases in choline acetyltransferase, NGF receptor and size of medial septum cholinergic neuronsBrain Res. 505: 29 - 38.
6. Lindh, B., et al. (1986) Immunohistochemical demonstration of choline acetyltransferase-immunoreactive preganglionic nerve fibers in guinea pig autonomic ganglia. Proc Natl Acad Sci U S A.83: 5316 - 5320.
7. Hagg, T. et al. (1992) Ciliary neurotrophic factor prevents neuronal degeneration and promotes low affinity NGF receptor expression in the adult rat CNSNeuron 8: 145-158.