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Cdc2 (pTyr15) Antibody

Cdc2 (pTyr15) Antibody gallery image 1

Western blot of human T47D cells showing phosphospecific immunolabeling of the ~34 kDa cdc 2 protein phosphoylated at Tyr15. In lane 2, cells were treated with EGF leading to dephosphorylation of Tyr15

  • Cdc2 (pTyr15) Antibody thumbnail image 1
  • Rabbit anti Cdc2 (pTyr15)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP895WB*datasheet pdfdatasheet pdf0.1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti phosphorylated CDC2 antibody recognizes Cyclin-dependent kinase 1, also known as Cell division control protein 2 homolog, CDC2, CDK1 or p34 protein kinase. Rabbit anti phosphorylated CDC2 antibody recognizes CDC2 only when phosphorylated at Tyr15.

      CDC2 is a serine kinase which plays an important role in the regulation of the cell cycle, mediating progression from G2 to M phase. Phosphorylation of tyrosine 15 results in inactivation of CDC2.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting(1)1/1000
      For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM Sodium Orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP895 detects a band of approximately 34kDa in human T47D cell lysates.
    • Instructions For Use

    Additional Cdc2 (pTyr15) Antibody Formats

    Formats Applications Sizes available
    Cdc2 (pTyr15) Antibody : Purified WB* 0.1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Cdc2 (pTyr15) Antibody thumbnail image 1

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