CD5 Antibody | OX-19

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CD5 Antibody | OX-19 gallery image 1

Staining of rat splenocytes with Mouse anti Rat CD5:FITC (MCA52F)

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CD5 Antibody | OX-19 gallery image 2

Staining of rat lymph node cells with Mouse anti Rat CD5: FITC (MCA52FB)

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  • CD5 Antibody | OX-19 thumbnail image 1
  • CD5 Antibody | OX-19 thumbnail image 2
  • Mouse anti Rat CD5
  • Mouse anti Rat CD5:Alexa Fluor® 488
  • Mouse anti Rat CD5
  • Mouse anti Rat CD5:FITC
  • Mouse anti Rat CD5
  • Mouse anti Rat CD5:Alexa Fluor® 647
  • Mouse anti Rat CD5:FITC
  • Mouse anti Rat CD5:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    OX-19
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA52GAC, F, IP, P *datasheet pdfdatasheet pdf0.1 mg
    MCA52GA
    MCA52FTFdatasheet pdfdatasheet pdf0.1 mg
    MCA52FT
    MCA52RC, F, IP, P *datasheet pdfdatasheet pdf0.25 mg
    MCA52R
    MCA52FBFdatasheet pdfdatasheet pdf0.5 mg
    MCA52FB
    MCA52GC, F, IP, P *datasheet pdfdatasheet pdf1 mg
    MCA52G
    MCA52A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA52A488
    MCA52A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA52A647
    MCA52FAFdatasheet pdfdatasheet pdf50 µg
    MCA52FA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD5 antibody, clone OX-19 recognizes the rat CD5 cell surface antigen, a 69kD glycoprotein expressed by T cells, thymocytes and a subset of B cells.

      Mouse anti Rat CD5 antibody, clone OX-19 has been reported as being suitable for use on periodate-lysine paraformaldehyde (PLP) fixed paraffin embedded tissue (Whiteland et al. 1995).

      Mouse anti Rat CD5 antibody, clone OX-19 is routinely tested in flow cytometry on rat splenocytes.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by ion exchange chromatography
      Purified IgG prepared by affinity chromatography on Protein G
      Pack Size: 50 µg, 0.1 mgPurified IgG prepared by ion exchange chromatography
      Pack Size: 0.5 mgPurified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
      Pack Size: 50 µg, 0.1 mgPurified IgG prepared by ion exchange chromatography
      Pack Size: 0.5 mgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 50 µg, 0.1 mgPurified IgG prepared by ion exchange chromatography
      Pack Size: 0.5 mgPurified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Rat thymocyte glycoproteins
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 1 mg, 0.1 mgIgG concentration 1.0mg/ml
      Pack Size: 0.25 mgIgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      Pack Size: 1 mg, 0.1 mgIgG concentration 1.0mg/ml
      Pack Size: 0.25 mgIgG concentration 1.0 mg/ml
      Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 1 mg, 0.1 mgIgG concentration 1.0mg/ml
      Pack Size: 0.25 mgIgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunised BALB/c mouse were fused with cells from the NS1 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      fully spanning the plasma membrane
      glycoprotein binding
      integral to membrane
      scavenger receptor activity
      regulation of signal transduction
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/100
      Immunohistology - Frozen
      Immunoprecipitation
      Immunohistology - Paraffin(1)
      (1)
      The use of PLP fixation is recommended for optimal results.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/100
      Immunohistology - Frozen
      Immunoprecipitation
      Immunohistology - Paraffin(1)
      (1)
      The use of PLP fixation is recommended for optimal results.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      1/100Pack Size: 0.5 mg
      NeatPack Size: 0.1 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/100
      Immunohistology - Frozen
      Immunoprecipitation
      Immunohistology - Paraffin(1)
      (1)
      The use of PLP fixation is recommended for optimal results.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      1/100Pack Size: 0.5 mg
      NeatPack Size: 0.1 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      1/100Pack Size: 0.5 mg
      NeatPack Size: 0.1 mg

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their particular system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD5 Antibody Formats

    Formats Clone Applications Sizes available
    CD5 Antibody : Alexa Fluor® 488 OX-19 F 100 Tests/1ml
    CD5 Antibody : Purified OX-19 C, F, IP, P * 0.1 mg | 0.25 mg | 1 mg
    CD5 Antibody : FITC OX-19 F 0.5 mg | 50 µg | 0.1 mg
    CD5 Antibody : Alexa Fluor® 647 OX-19 F 100 Tests/1ml
    • Copyright © 2016 Bio-Rad

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Frozen

              References

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