CD45R Antibody | HIS24 (6B6)

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  • Mouse anti Rat CD45R
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    • Product Type
      Monoclonal Antibody
    • Clone
      HIS24 (6B6)
    • Isotype
      IgG2b
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA1326C*, Fdatasheet pdfdatasheet pdf2 ml
      MCA1326
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Mouse anti Rat CD45R antibody, clone HIS24 recognizes the high molecular weight form of the leucocyte common antigen (LCA) or CD45. CD45R is expressed on mature and precursor B lymphocytes, and to some extent on marginal zone B cells and a small subset of T cells.

        Mouse anti Rat CD45R antibody, clone HIS24 is routinely used as a pan-B-cell marker for immunohistochemical studies on brain tissue (Ali et al. 2005), pancreas (Homo-Delarche et al. 2006), pulmonary tissue (Foxwell et al. 2001) and in flow cytometry on bone marrow cells (Prakapas et al. 1993).
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1 / 5
        Immunohistology - Paraffin
        Immunohistology - Resin
        Immunohistology - Frozen(1)1 / 40
        (1)
        The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
      • ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional CD45R Antibody Formats

      Formats Clone Applications Sizes available
      CD45R Antibody : S/N HIS24 (6B6) C*, F 2 ml

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
        STAR117A
        Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
        STAR87A
        Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
        STAR117D488GA
        Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
        STAR117D549GA
        Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
        STAR117D649GA
        Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
        STAR117D680GA
        Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
        STAR117D800GA
        Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
        STAR8D800GA
        Human anti Mouse IgG2b:FITCHCA038F0.1 mgF
        HCA038F
        Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
        STAR117F
        Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
        STAR70
        Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
        STAR120F
        Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
        STAR9B
        Human anti Mouse IgG2b:HRPHCA038P0.1 mgE
        HCA038P
        Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
        STAR117P
        Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
        STAR77
        Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
        STAR120P
        Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
        STAR13B
        Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
        STAR87P
        Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
        STAR12A
        Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
        STAR76

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG2b Negative ControlMCA691100 TestsF
          MCA691

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                • Application NameReference Images
                  Immunohistology - Frozen

                References

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