CD45 Antibody | OX-29

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  • Mouse anti Rat CD45
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    OX-29
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA589RF, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA589R
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Rat CD45 antibody, clone OX-29 recognizes rat CD45, also known as Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (LCA) or T200, expressed on all rat leukocytes. CD45 is a single pass, type I transmembrane glycoprotein of ~180-240kDa depending on the isoform and degree of glycosylation. Rat CD45 contains two intracellular tyrosine-protein phosphatase domains and at least two extracellular fibronectin type-III domains (Uniprot Acc no: P04157), although the existence of a third similar domain has previously been proposed (Symons et al. 1999).

      Mouse anti Rat CD45 antibody, clone OX-29 has been reported as recognizing a monomorphic determinant present on all allogenic forms of the rat CD45 antigen (Symons and Barclay 2000). Epitope mapping further indicates that the OX-29 clone binds to a determinant located within the membrane distal two domains of CD45 (Symons, et al. 1999).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD45 Antibody Formats

    Formats Clone Applications Sizes available
    CD45 Antibody : Purified OX-29 F, IP 0.25 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA1210100 TestsF
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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