CD45.2 Antibody | HIS41

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  • Mouse anti Rat CD45.2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    HIS41
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA473SC *, F, IPdatasheet pdfdatasheet pdf2 ml
    MCA473S
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD45.2 antibody, clone HIS41 recognizes the rat RT7.2 alloantigen - it does not react with RT7.1. RT7.2 is expressed on the surface of all peripheral leucocytes, as well as on bone marrow cells of lymphoid and myeloid lineage. Immunoprecipitation studies demonstrate a band of ~180 kDa from thymocytes, and ~190-220 kDa from T and B cells. Sequential immunoprecipitation studies have revealed that HIS41 recognises a determinant of the leucocyte common antigen (LCA, CD45).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Paraffin
      Immunoprecipitation
      Immunohistology - Frozen(1)Neat1/10
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD45.2 Antibody Formats

    Formats Clone Applications Sizes available
    CD45.2 Antibody : S/N HIS41 C *, F, IP 2 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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              References

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