Mouse anti Rat CD4:FITC/CD25:RPE dual color reagent is a dual colour reagent designed to allow the simultaneous enumeration of cells expressing the rat CD4 and CD25 cell surface antigens.
Clone W3/25 recognises rat CD4, a T cell differentiation antigen expressed by helper T lymphocytes and weakly by monocytes. Clone OX-39 recognises rat CD25, a ~55 kDa glycoprotein expressed by activated T lymphocytes.
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cell in 100ul.