DC042 is a dual colour reagent recognising rat CD3 and CD8 cell surface antigens.
Clone IF4 recognises rat CD3, a cell surface glycoprotein expressed by mature T lymphocytes. Clone OX8 recognises rat CD8, a cell surface glycoprotein expressed by thymocytes and by the suppressor/cytotoxic subset of peripheral blood T cells.
CD8 expression has also been reported upon rat macrophages.
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.