CD3/CD4 | IF4 : W3/25

CD3/CD4 | IF4 : W3/25 gallery image 1

Staining of rat spleen cells with Mouse anti Rat CD3:FITC/CD4:RPE (DC041)

CD3/CD4 | IF4 : W3/25 gallery image 2

Published customer image:
Dual color reagent Mouse anti Rat CD3 and CD4 antibodies (DC041) used for staining of lymphocyte subsets by flow cytometry
Image caption:
Mature CD4 or CD8 positive T lymphocytes in the peripheral blood of Rag1 mutant rats. A Absence of CD4+/CD8+ lymphocytes in wild type and Rag1 mutant rats. B and C Reduction of CD4+/CD3+ and CD8+/CD3+ T cells. D and E While there are no differences in the population of CD25+/CD4+ T cells the number of CD25+/CD8+ lymphocytes was reduced. orange: autofluorescent cells; green: FITC positive lymphocytes; blue: PE positive lymphocytes.

From: Zschemisch NH, Glage S, Wedekind D, Weinstein EJ, Cui X, Dorsch M, Hedrich HJ.
Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.
BMC Immunol. 2012 Nov 8;13:60.

  • CD3/CD4 | IF4 : W3/25 thumbnail image 1
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  • Mouse anti Rat CD3:FITC/CD4:RPE
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  • Product Type
    Dual Color Reagent
  • Clone
    IF4 : W3/25
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    DC041Fdatasheet pdfdatasheet pdf100 Tests
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Dual colour reagent Mouse anti Rat CD3 and CD4 antibodies is a combination of monoclonal antibodies recognising rat CD3 and CD4 cell surface antigens.

      Mouse anti Rat CD3 antibody, clone IF4 recognizes rat CD3, a cell surface glycoprotein expressed by mature T lymphocytes.

      Mouse anti Rat CD4 antibody, clone W3/25 recognises rat CD4, a ~55 kDa glycoprotein expressed by helper T lymphocytes, thymocytes and macrophages.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cell in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD3/CD4 Formats

    Formats Clone Applications Sizes available
    CD3/CD4 : FITC/RPE IF4 : W3/25 F 100 Tests
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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