CD3/CD4/CD8 | 1F4 / W3/25 / OX-8

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  • anti Rat CD3:FITC/CD4:RPE/CD8:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Triple Color Reagent
  • Clone
    1F4 / W3/25 / OX-8
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    TC018Fdatasheet pdfdatasheet pdf50 Tests
    TC018
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Anti Rat CD3:FITC/CD4:RPE/CD8:Alexa Fluor® 647 is a triple color reagent which can be used for the phenotyping and enumeration of mature rat T-cell populations.

      Antibodies produced by clone 1F4 recognize rat CD3, a 25 kDa cell surface antigen which is expressed by mature rat T-cells.

      Antibodies produce by clone W3/25 recognize the rat CD4 cell surface glycoprotein, a 55 kDa molecule expressed by helper T cells and weakly by monocytes.

      Antibodies produced by clone OX-8 recognize the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD3/CD4/CD8 Formats

    Formats Clone Applications Sizes available
    CD3/CD4/CD8 : 3 Color 1F4 / W3/25 / OX-8 F 50 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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