Anti Rat CD3:FITC/CD25:RPE/RT1B:Alexa Fluor® 647 is a triple color reagent which can be used for the phenotyping and enumeration of rat lymphocyte populations.
Antibodies produced by clone 1F4 recognize rat CD3, a 25 kDa cell surface antigen which is expressed by mature rat T-cells.
Antibodies produced by clone OX-39 react with the alpha chain of rat CD25, a 55 kDa type I membrane glycoprotein otherwise known as the IL-2 receptor alpha. In rats CD25 is expressed by activated T cells but not resting lymphocytes.
Antibodies produced by clone OX-6 react with a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages.
Triple Color combination consisting of FITC, RPE and Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or firstname.lastname@example.org
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul