CD3/CD25/RT1B | 1F4 / OX-39 / OX-6

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  • anti Rat CD3:FITC/CD25:RPE/RT1B:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Triple Color Reagent
  • Clone
    1F4 / OX-39 / OX-6
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    TC020Fdatasheet pdfdatasheet pdf50 Tests
    TC020
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Anti Rat CD3:FITC/CD25:RPE/RT1B:Alexa Fluor® 647 is a triple color reagent which can be used for the phenotyping and enumeration of rat lymphocyte populations.

      Antibodies produced by clone 1F4 recognize rat CD3, a 25 kDa cell surface antigen which is expressed by mature rat T-cells.

      Antibodies produced by clone OX-39 react with the alpha chain of rat CD25, a 55 kDa type I membrane glycoprotein otherwise known as the IL-2 receptor alpha. In rats CD25 is expressed by activated T cells but not resting lymphocytes.

      Antibodies produced by clone OX-6 react with a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Triple Color combination consisting of FITC, RPE and Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
    • Reconstitution
      Reconstitute with 0.5 ml distilled water
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      5%Sucrose
    • Purity
    • Approx. Protein Concentrations
    • Antibody Isotypes
      CD3 FITC reagent: Mouse IgM
      CD25 RPE reagent: Mouse IgG1
      RT1B A647 reagent: Mouse IgG1
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of reconstitution
    • GO Terms
      protein transport
      interleukin-2 receptor activity
      regulation of apoptosis
      integral to membrane
      establishment or maintenance of cell polarity
      drug binding
      negative regulation of immune response
      inflammatory response to antigenic stimulus
      cell surface receptor linked signaling pathway
      positive thymic T cell selection
      negative regulation of defense response to virus
      protein heterodimerization activity
      positive regulation of T cell differentiation
      transmembrane receptor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD3/CD25/RT1B Formats

    Formats Clone Applications Sizes available
    CD3/CD25/RT1B : 3 Color 1F4 / OX-39 / OX-6 F 50 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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