CD28 Antibody | JJ319

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CD28 Antibody | JJ319 gallery image 1

Staining of Rat lymph node cells with Mouse anti Rat CD28:RPE (MCA1331PE)

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CD28 Antibody | JJ319 gallery image 2

Staining of rat thymus cells with Mouse anti Rat CD28:FITC (MCA1331F)

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  • CD28 Antibody | JJ319 thumbnail image 1
  • CD28 Antibody | JJ319 thumbnail image 2
  • Mouse anti Rat CD28:Alexa Fluor® 647
  • Mouse anti Rat CD28:Alexa Fluor® 488
  • Mouse anti Rat CD28:Low Endotoxin
  • Mouse anti Rat CD28:FITC
  • Mouse anti Rat CD28:RPE
  • Mouse anti Rat CD28
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    JJ319
  • Isotype
    IgG1
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1331FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1331F
    MCA1331F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA1331
    MCA1331ELF, FN, IPdatasheet pdfdatasheet pdf0.5 mg
    MCA1331EL
    MCA1331PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1331PE
    MCA1331A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1331A647
    MCA1331A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1331A488
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD28 antibody, clone JJ319 recognizes the rat cell surface antigen CD28, a ~90 kDa type I transmembrane glycoprotein, expressed by the majority of T lymphocytes and a subset of NK cells.

      CD28 is an essential costimulatory receptor for T cell activation and a modulator of T-cell cytokine production. Ligands of CD28 include CD80 (B7-1), CD86 (B7-2) and P13-kinase.

      >Mouse anti Rat CD28 antibody, clone JJ319 has been identified as a highly effective co-stimulator of T cell proliferation and IL-2 secretion by resting T cells (Tacke et al. 1995).
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
    • Immunogen
      Mice were immunized with rat CD28 transfected A20J cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mive were fused with cells from the X63-Ag 8.653 mouse myeloma.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Storage in frost-free freezers is not recommended.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Storage in frost-free freezers is not recommended.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      membrane raft
      T cell costimulation
      integral to membrane
      protein homodimerization activity
      positive regulation of mitosis
      coreceptor activity
      positive regulation of interleukin-2 biosynthetic process
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/2
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Functional Assays
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/20
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD28 Antibody Formats

    Formats Clone Applications Sizes available
    CD28 Antibody : RPE JJ319 F 100 Tests
    CD28 Antibody : FITC JJ319 F 0.1 mg
    CD28 Antibody : Alexa Fluor® 488 JJ319 F 100 Tests/1ml
    CD28 Antibody : Alexa Fluor® 647 JJ319 F 100 Tests/1ml
    CD28 Antibody : Low Endotoxin JJ319 F, FN, IP 0.5 mg
    CD28 Antibody : Purified JJ319 F, IP 0.25 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA1209A647100 Tests/1mlF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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