CD1d Antibody | WTH1

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CD1d Antibody | WTH1 gallery image 1

Immunoperoxidase staining of LEW rat spleen follicle with Mouse anti Rat CD1d (MCA5799)

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CD1d Antibody | WTH1 gallery image 2

Staining of rat thymocytes with Mouse anti Rat CD1d:FITC (MCA5799F)

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  • CD1d Antibody | WTH1 thumbnail image 1
  • CD1d Antibody | WTH1 thumbnail image 2
  • Mouse anti Rat CD1d
  • Mouse anti Rat CD1d:FITC
  • Mouse anti Rat CD1d
  • Mouse anti Rat CD1d:FITC
  • Mouse anti Rat CD1d:Biotin
  • Mouse anti Rat CD1d:Biotin
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  • Product Type
    Monoclonal Antibody
  • Clone
    WTH1
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5799FFdatasheet pdfdatasheet pdf0.1 mg
    MCA5799F
    MCA5799GAC, E, F, FN*, IP, WB*datasheet pdfdatasheet pdf0.1 mg
    MCA5799GA
    MCA5799BC, E, F, FN*, IP, WB*datasheet pdfdatasheet pdf0.1 mg
    MCA5799B
    MCA5799C, E, F, FN*, IP, WB*datasheet pdfdatasheet pdf0.25 mg
    MCA5799
    MCA5799FTFdatasheet pdfdatasheet pdf25 µg
    MCA5799FT
    MCA5799BTC, E, F, FN*, IP, WB*datasheet pdfdatasheet pdf25 µg
    MCA5799BT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD1d antibody, clone WTH1 recognizes rat CD1d, a glycoprotein expressed on cortical thymocytes, certain T-cell leukaemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T-cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines.

      Clone WTH1 recognizes a different, non-overlapping epitope to clone WTH2 and binds with high affinity to the mature CD1d heavy chain independently of beta-2-microglobulin. Mouse anti Rat CD1d antibody, clone WTH1 efficiently blocks rat and mouse invariant natural killer T cell (iNKT cell) response to a-GalCer (an antigen for iNKT cells) and has a slightly higher affinity to native CD1d than clone WTH2.

      Clone WTH1 cross reacts weakly with mouse tissue in IHC and western blotting, but reacts strongly with both rat and mouse antigens in flow cytometry and immunoprecipitation.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
      Pack Size: 25 µg0.02% Sodium Azide (NaN3)
      0.02% Sodium Azide (NaN3)
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
      Pack Size: 25 µg0.02% Sodium Azide (NaN3)
      0.02% Sodium Azide (NaN3)
      0.02% Sodium Azide (NaN3)
    • Immunogen
      Rat CD1d-transduced M12.4.1.C3 cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
      IgG concentration 0.5mg/ml
      IgG concentration 0.5mg/ml
      IgG concentration 0.5mg/ml
      IgG concentration 0.5mg/ml
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      late endosome
      positive regulation of NK T cell activation
      endogenous lipid antigen binding
      positive regulation of interleukin-4 biosynthetic process
      positive regulation of interferon-gamma biosynthetic process
      integral to membrane
      protein binding
      positive regulation of interleukin-2 biosynthetic process
      positive thymic T cell selection
      antigen processing and presentation, exogenous lipid antigen via MHC class Ib
      positive regulation of NK T cell differentiation
      lysosomal membrane
      innate immune response
      MHC class I protein complex
      early endosome
      positive regulation of T cell mediated cytotoxicity
      external side of plasma membrane
      endosome membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/2,000
      Immunohistology - Frozen
      Immunoprecipitation
      Functional Assays(1)
      Western Blotting(2)
      (1)
      This antibody contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
      (2)
      Clone WTH1 recognizes CD1d under non-reducing conditions, see Monzon-Casanova, E. et al. for details.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1,000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/2,000
      Immunohistology - Frozen
      Immunoprecipitation
      Functional Assays(1)
      Western Blotting(2)
      (1)
      This antibody contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
      (2)
      Clone WTH1 recognizes CD1d under non-reducing conditions, see Monzon-Casanova, E. et al. for details.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1,000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/1,000
      Immunohistology - Frozen
      Immunoprecipitation
      Functional Assays(1)
      Western Blotting(2)
      (1)
      This antibody contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
      (2)
      Clone WTH1 recognizes CD1d under non-reducing conditions, see Monzon-Casanova, E. et al. for details.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/1,000
      Immunohistology - Frozen
      Immunoprecipitation
      Functional Assays(1)
      Western Blotting(2)
      (1)
      This antibody contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
      (2)
      Clone WTH1 recognizes CD1d under non-reducing conditions, see Monzon-Casanova, E. et al. for details.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD1d Antibody Formats

    Formats Clone Applications Sizes available
    CD1d Antibody : Biotin WTH1 C, E, F, FN*, IP, WB* 0.1 mg | 25 µg
    CD1d Antibody : Purified WTH1 C, E, F, FN*, IP, WB* 0.1 mg | 0.25 mg
    CD1d Antibody : FITC WTH1 F 25 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

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              References

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