CD161 Antibody | 10/78

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CD161 Antibody | 10/78 gallery image 1

Staining of rat peripheral blood lymphocytes with Mouse anti RatT CD161:Alexa Fluor® 647 (MCA1427A647)

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CD161 Antibody | 10/78 gallery image 2

Published customer image:
Phycoerythrin conjugated Mouse anti Rat CD161 antibody, clone 10/78 (MCA1427PE) used for the identification of NK cells in rat blood samples by flow cytometry.
Image caption:
Graft survival in NK cell depleted recipients. A: 3-week-old (young) Lewis rats were injected twice with a NK cell depleting antibody (3.2.3). Blood was taken and analyzed by flow cytometry for the presence of CD3+ and CD161+ cells. Intraperitoneal injection of 3.2.3 lead to complete absence of CD161+ NK/NKT cells and did not affect cell numbers of CD3+CD161- T cells. FACS data shown are representative for control animals that received an isotypic antibody (NK+) or 3.2.3-treated rats (NK-). B: After injections of either isotyp controls (NK+) or 3.2.3 (NK-) allogeneic penetrating keratoplasty was performed and the grafts were assessed clinically until rejection occurred. Albeit all recipients of both allogeneic groups rejected, animals of group NK- showed a statistically significantly delay of immunological allograft failure (p<0.01). None of the syngeinic controls (groups syn.NK+ and syn.NK-, respectively) rejected. C: NK cells were depleted in 10-week-old (old) Lewis rats (NK-) or isotypic antibody was injected (NK+). NK cell depletion had no effect on graft survival rates.

From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, Reinhard T. NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 2010 Oct 2;16:1928-35.

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  • CD161 Antibody | 10/78 thumbnail image 1
  • CD161 Antibody | 10/78 thumbnail image 2
  • Mouse anti Rat CD161:FITC
  • Mouse anti Rat CD161:FITC
  • Mouse anti Rat CD161:RPE
  • Mouse anti Rat CD161:Preservative Free
  • Mouse anti Rat CD161:Alexa Fluor® 647
  • Mouse anti Rat CD161
  • Mouse anti Rat CD161
  • Mouse anti Rat CD161:FITC
  • Mouse anti Rat CD161:Alexa Fluor® 488
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  • Product Type
    Monoclonal Antibody
  • Clone
    10/78
  • Isotype
    IgG1
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1427FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1427F
    MCA1427GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA1427GA
    MCA1427C, F, IP, R, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA1427
    MCA1427FBFdatasheet pdfdatasheet pdf0.5 mg
    MCA1427FB
    MCA1427XZC, F, FN, IP, R, WBdatasheet pdfdatasheet pdf1 mg
    MCA1427XZ
    MCA1427PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1427PE
    MCA1427A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1427A647
    MCA1427A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1427A488
    MCA1427FAFdatasheet pdfdatasheet pdf50 µg
    MCA1427FA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD161 antibody, clone 10/78 recognizes the rat Killer cell lectin-like receptor subfamily B protein, also known as NKR-PI or CD161. CD161 is a 233 amino acid ~60 kDa type II single pass protein containing a single C-type lectin domain. CD161 is expressed on rat NK cells and T cell subpopulations. CD161 exists in 2 forms NKR-PIa and NKR-PIb, clone 10/78  recognizes both forms of CD161 (Li et al. 2003). Clone 10/78 competes with another anti CD161 clone, 3.2.3 for binding to antigen.

      Mouse anti Rat CD161 antibody, clone 10/78 has been successfully employed for the in vivo depletion of rat NK cells in an experimental obesity model (Wrann et al. 2010).
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      None present
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Purified splenic NK cells from the LEW rat strain.
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.1 mg, 50 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 0.1 mg, 50 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      Pack Size: 0.1 mg, 50 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.Ag8653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Neat Pack Size: 0.1 mg
      1/25 Pack Size: 0.5 mg
      1/10Pack Size: 0.1 mg
      1/100Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Neat Pack Size: 0.1 mg
      1/25 Pack Size: 0.5 mg
      1/10Pack Size: 0.1 mg
      1/100Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Functional Assays
      Immunohistology - Frozen
      Immunoprecipitation
      Radioimmunoassays
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
      Radioimmunoassays
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
      Radioimmunoassays
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Neat Pack Size: 0.1 mg
      1/25 Pack Size: 0.5 mg
      1/10Pack Size: 0.1 mg
      1/100Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Sugggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD161 Antibody Formats

    Formats Clone Applications Sizes available
    CD161 Antibody : Alexa Fluor® 488 10/78 F 100 Tests/1ml
    CD161 Antibody : FITC 10/78 F 50 µg | 0.5 mg | 0.1 mg
    CD161 Antibody : RPE 10/78 F 100 Tests
    CD161 Antibody : Purified 10/78 C, F, IP, R, WB 0.25 mg | 0.1 mg
    CD161 Antibody : Alexa Fluor® 647 10/78 F 100 Tests/1ml
    CD161 Antibody : Preservative Free 10/78 C, F, FN, IP, R, WB 1 mg
    • Copyright © 2016 Bio-Rad

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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