CD122 Antibody | L316

CD122 Antibody | L316 gallery image 1

Published customer image:
Mouse anti Human CD122 antibody (MCA2089) used to block dimeric IL-2 mediated apoptosis in human vascular smooth muscle cells in vitro
Image caption:
Dimeric IL-2-mediated cell death is blocked by antibodies recognizing IL-2 or IL-2Rβ.(A) Murine dimeric IL-2, from tissues, at 100 ng/ml was incubated with increasing concentrations of anti-murine IL-2 antibodies (clone S4B6) or an isotype control (20 7mu;g/ml) for 30 minutes at 37°C then added to cultured VSMC. Cell death at 1 hour post-dimer addition was assessed by CellTox Green as previously described. “Media” indicates cells cultured in the absence of dimer or antibody. (B) VSMCs were pre-incubated for 15 minutes with increasing concentrations of anti- murine IL-2Rβ antibodies or an isotype control (50 μg/ml). Dimeric IL-2 at 100 ng/ml was added and cell death assessed using CellTox Green. Cell death at 20 minutes post-dimer addition is shown. (C) Rat tubular epithelial cells were pre-incubated for 15 minutes with 10 7mu;g/ml anti-rat IL-2Rβ antibodies or an isotype control (10 μg/ml). Dimeric IL-2, from EL-4 media, at 10 ng/ml was added and cell death was assessed at 20 minutes post-dimer addition as in B. The results shown are the mean ± SD of duplicate wells, and are each representative of 5 separate experiments. Percent lysis is significantly different across the increasing concentrations of antibodies in Figures A and B (A, p?=?6.6×10-6; B, p?=?5.8×10-5, by ANOVA). In C, treatment with anti-CD122 is significantly different than isotype control (p<.05, one-sided t test).

From: Wrenshall LE, Clabaugh SE, Cool DR, Arumugam P, Grunwald WC, Smith DR, et al. (2014)
Identification of a Cytotoxic Form of Dimeric Interleukin-2 in Murine Tissues.
PLoS ONE 9(7): e102191.

  • CD122 Antibody | L316 thumbnail image 1
  • Mouse anti Rat CD122:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2089FF*datasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Rat CD122 antibody, clone L316 recognizes CD122 the 80 kDa Beta subunit of IL-2R, which is shared by the IL-15 receptor. The interleukin-2 receptor (IL-2R) is composed of three subunits, namely alpha, beta and gamma. In peripheral lymphoid tissues of adult rats, CD122 is primarily expressed by NK cells and a subset of T lymphocytes.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Rat CRNK cells and rat IL-2R beta cDNA-transfected mouse L929 cell line.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      natural killer cell activation
      integral to membrane
      interleukin-2 receptor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      An incubation time of 4 hours with MCA2089XZ is recommended for optimum staining, see Park et al.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD122 Antibody Formats

    Formats Clone Applications Sizes available
    CD122 Antibody : FITC L316 F* 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA1209F0.1 mgF

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays


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