Mouse anti Rat CD122 antibody, clone L316 recognizes CD122 the 80 kDa Beta subunit of IL-2R, which is shared by the IL-15 receptor. The interleukin-2 receptor (IL-2R) is composed of three subunits, namely alpha, beta and gamma. In peripheral lymphoid tissues of adult rats, CD122 is primarily expressed by NK cells and a subset of T lymphocytes.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
An incubation time of 4 hours with MCA2089XZ is recommended for optimum staining, see Park et al.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
1. Park JH et al. (1996) Identification and cellular distribution of the rat interleukin-2 receptor beta chain: induction of the IL-2R alpha- beta+ phenotype by major histocompatibility complex class I recognition during T cell development in vivo and by T cell receptor stimulation of CD4+8+ immature thymocytes in vitro.Eur J Immunol. 26 (10): 2371-5.
2. Wrenshall, L.E. et al. (2014) Identification of a cytotoxic form of dimeric interleukin-2 in murine tissues.PLoS One. 9: e102191.