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Mouse anti Human CD122 antibody (MCA2089) used to block dimeric IL-2 mediated apoptosis in human vascular smooth muscle cells in vitro
Dimeric IL-2-mediated cell death is blocked by antibodies recognizing IL-2 or IL-2Rβ.(A) Murine dimeric IL-2, from tissues, at 100 ng/ml was incubated with increasing concentrations of anti-murine IL-2 antibodies (clone S4B6) or an isotype control (20 7mu;g/ml) for 30 minutes at 37°C then added to cultured VSMC. Cell death at 1 hour post-dimer addition was assessed by CellTox Green as previously described. “Media” indicates cells cultured in the absence of dimer or antibody. (B) VSMCs were pre-incubated for 15 minutes with increasing concentrations of anti- murine IL-2Rβ antibodies or an isotype control (50 μg/ml). Dimeric IL-2 at 100 ng/ml was added and cell death assessed using CellTox Green. Cell death at 20 minutes post-dimer addition is shown. (C) Rat tubular epithelial cells were pre-incubated for 15 minutes with 10 7mu;g/ml anti-rat IL-2Rβ antibodies or an isotype control (10 μg/ml). Dimeric IL-2, from EL-4 media, at 10 ng/ml was added and cell death was assessed at 20 minutes post-dimer addition as in B. The results shown are the mean ± SD of duplicate wells, and are each representative of 5 separate experiments. Percent lysis is significantly different across the increasing concentrations of antibodies in Figures A and B (A, p?=?6.6×10-6; B, p?=?5.8×10-5, by ANOVA). In C, treatment with anti-CD122 is significantly different than isotype control (p<.05, one-sided t test).
From: Wrenshall LE, Clabaugh SE, Cool DR, Arumugam P, Grunwald WC, Smith DR, et al. (2014)
Identification of a Cytotoxic Form of Dimeric Interleukin-2 in Murine Tissues.
PLoS ONE 9(7): e102191.